Recombinant Human Malate Dehydrogenase 2 | MyBioSource MBS144141 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Malate Dehydrogenase 2

catalog :

MBS144141

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144141

products type :

Recombinant Protein

products full name :

Recombinant Human Malate Dehydrogenase 2

products short name :

Malate Dehydrogenase 2

products name syn :

MDH2 Human; Malate Dehydrogenase 2 Human Recombinant; M-MDH; MDH; MOR1

other names :

malate dehydrogenase, mitochondrial isoform 2; Malate dehydrogenase, mitochondrial; malate dehydrogenase, mitochondrial; malate dehydrogenase 2, NAD (mitochondrial)

products gene name :

MDH2

other gene names :

MDH2; MDH2; MDH; MOR1; M-MDH; MGC:3559

uniprot entry name :

MDHM_HUMAN

host :

E Coli

sequence length :

296

sequence :

MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGCDVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAELKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVVECSFVKSQET ECTYFSTPLL LGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK.

purity :

Greater than 95.0% as determined by SDS-PAGE.

form :

The MDH2 1mg/ml protein solution contains 20mM Tris-HCl pH-7.5 and 10% glycerol. Sterile Filtered colorless solution.

storage stability :

MDH2 although stable 4 degree C for 4 weeks, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

other info2 :

Biological Activity: Specific activity is > 30 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 at 25 degree C.

products categories :

ENZYMES; Enzymes; Dehydrogenase

products description :

Description: MDH2 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 335 amino acids (25-338 a.a.) and having a molecular mass of 35.2 kDa. The MDH2 is fused to a 21 amino acid His tag at N-terminus and purified by conventional chromatography. Introduction: MDH2 catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. MDH2 is localized to the mitochondria and takes part in the malate-aspartate shuttle that functions in the metabolic coordination between cytosol and mitochondria. MDH2 is highy expressed in the adrenal system, small intestine, heart and pancreas.

ncbi gi num :

538919685

ncbi acc num :

NP_001269332.1

ncbi gb acc num :

NM_001282403.1

uniprot acc num :

P40926

ncbi mol weight :

30,875 Da

ncbi pathways :

Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (855811); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (468202); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (413348); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (468204); Citric Acid Cycle (TCA Cycle) Pathway (105919); Cysteine And Methionine Metabolism Pathway (104488)

ncbi summary :

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

uniprot summary :

MDH2: mitochondrial malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. May play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Protein type: Mitochondrial; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 1.1.1.37; Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase. Chromosomal Location of Human Ortholog: 7cen-q22. Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; plasma membrane; nucleus. Molecular Function: protein self-association; L-malate dehydrogenase activity; malate dehydrogenase (NADP+) activity. Biological Process: cellular metabolic process; malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; cellular carbohydrate metabolic process; pathogenesis; internal protein amino acid acetylation; gluconeogenesis

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

5015

more info or order :

MyBioSource product webpage