Recombinant Human Enoyl CoA Hydratase, Short chain, 1, Mitochondrial | MyBioSource MBS144128 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Enoyl CoA Hydratase, Short chain, 1, Mitochondrial

catalog :

MBS144128

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS144128

products type :

Recombinant Protein

products full name :

Recombinant Human Enoyl CoA Hydratase, Short chain, 1, Mitochondrial

products short name :

[Enoyl CoA Hydratase, Short chain, 1]

products name syn :

[ECHS1 Human; Enoyl CoA Hydratase, Short chain, 1, Mitochondrial Human Recombinant; Enoyl-CoA hydratase 1; SCEH]

other names :

[enoyl-CoA hydratase, mitochondrial; Enoyl-CoA hydratase, mitochondrial; enoyl-CoA hydratase, mitochondrial; enoyl Coenzyme A hydratase, short chain, 1, mitochondrial; enoyl-CoA hydratase 1; short-chain enoyl-CoA hydratase; enoyl CoA hydratase, short chain, 1, mitochondrial; Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH]

products gene name :

[ECHS1]

other gene names :

[ECHS1; ECHS1; SCEH; SCEH]

uniprot entry name :

ECHM_HUMAN

host :

E Coli

sequence length :

290

sequence :

MGSSHHHHHH SSGLVPRGSH M ASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ

purity :

Greater than 95.0% as determined by SDS-PAGE.

form :

The ECHS1 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH-8), 1mM DTT,0.1M NaCl and 20% glycerol. Sterile filtered colorless solution.

concentration :

1mg/ml

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

other info1 :

Volume: 1 ml

other info2 :

Physical Appearance: Sterile filtered colorless solution.

products categories :

ENZYMES; Enzymes; Hydratase

products description :

Description: ECHS1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 284 amino acids (28-290 a.a.) and having a molecular mass of 30.6kDa.ECHS1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: ECHS1 is part of the hydratase/isomerase superfamily. ECHS1 is localized in the mitochondrial matrix and Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that takes part in the second phase of the mitochondrial fatty acid beta-oxidation pathway.

ncbi gi num :

194097323

ncbi acc num :

NP_004083.3

ncbi gb acc num :

NM_004092.3

uniprot acc num :

P30084

ncbi pathways :

Beta Oxidation Of Butanoyl-CoA To Acetyl-CoA Pathway (106130); Beta Oxidation Of Decanoyl-CoA To Octanoyl-CoA-CoA Pathway (106127); Beta Oxidation Of Hexanoyl-CoA To Butanoyl-CoA Pathway (106129); Beta Oxidation Of Lauroyl-CoA To Decanoyl-CoA-CoA Pathway (106126); Beta Oxidation Of Octanoyl-CoA To Hexanoyl-CoA Pathway (106128); Butanoate Metabolism Pathway (83007); Butanoate Metabolism Pathway (391); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Fatty Acid Beta Oxidation Pathway (198865)

ncbi summary :

The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq, Jul 2008]

uniprot summary :

ECHS1: Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Belongs to the enoyl-CoA hydratase/isomerase family. Protein type: EC 4.2.1.17; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - butanoate; Lyase; Lipid Metabolism - fatty acid; Amino Acid Metabolism - lysine degradation; Lipid Metabolism - fatty acid elongation in mitochondria; Mitochondrial; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - tryptophan; Carbohydrate Metabolism - propanoate. Chromosomal Location of Human Ortholog: 10q26.2-q26.3. Cellular Component: mitochondrion; mitochondrial matrix. Molecular Function: protein binding; enoyl-CoA hydratase activity. Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process. Disease: Mitochondrial Short-chain Enoyl-coa Hydratase 1 Deficiency

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

2665

more info or order :

MyBioSource product webpage