protein

Recombinant Human Tumor Susceptibility Gene 101

MBS144073

0.002 mg

140 USD

Recombinant Protein

Recombinant Human Tumor Susceptibility Gene 101

Tumor Susceptibility Gene 101

TSG101 Human; Tumor Susceptibility Gene 101 Human Recombinant; TSG10; VPS23; TSG101; ESCRT-I complex subunit TSG101; Tumor susceptibility gene 101 protein

tumor susceptibility gene 101 protein; Tumor susceptibility gene 101 protein; tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101; tumor susceptibility protein; tumor susceptibility 101; ESCRT-I complex subunit TSG101

TSG101

TSG101; TSG101; TSG10; VPS23

TS101_HUMAN

E Coli

390

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAVS ESQLKKMVSK YKYRDLTVRE TVNVITLYKD LKPVLDSYVF NDGSSRELMN LTGTIPVPYR GNTYNIPICL WLLDTYPYNP PICFVKPTSS MTIKTGKHVD ANGKIYLPYL HEWKHPQSDL LGLIQVMIVV FGDEPPVFSR P.

Greater than 90% as determined by SDS-PAGE.

TSG101 protein solution (0.5mg/ml) containing 20mM Tris-HCl pH-8, 1mM DTT and 10% glycerol. Sterile filtered colorless solution.

0.5 mg/ml

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins

Description: TSG101 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids (1-145 a.a.) and having a molecular mass of 20.7 kDa. TSG101 protein is fused to a 36 amino acid His-Tag at N-terminus and purified by standard chromatography. Introduction: TSG101 is a member of apparently inactive homologs of ubiquitin-conjugating enzymes. TSG101 contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. TSG101 is involved in cell growth and differentiation and acts as a negative growth regulator. TSG101 in vitro steady-state expression is important for maintenance of genomic stability and cell cycle regulation. TSG101 mutations and alternative splicing occur in high rate in breast cancer and implicate that defects occur during breast cancer tumorigenesis and/or progression. TSG101 is a factor of the ESCRT-I complex, a monitor of vesicular trafficking process. TSG101 binds to ubiquitinated cargo proteins and is needed for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. TSG101 is needed for completion of cytokinesis and is involved in cell growth and differentiation.

5454140

NP_006283.1

NM_006292.3

Q99816

31,732 Da

Assembly Of The HIV Virion Pathway (106008); Budding And Maturation Of HIV Virion Pathway (106009); Disease Pathway (530764); ESCRT-I Complex Pathway (413473); ESCRT-I Complex Pathway (890624); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Endosomal Sorting Complex Required For Transport (ESCRT) Pathway (205244); Glucocorticoid Receptor Regulatory Network Pathway (138014); HIV Infection Pathway (105983)

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]

TSG101: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Nuclear receptor co-regulator; Cell cycle regulation. Chromosomal Location of Human Ortholog: 11p15. Cellular Component: multivesicular body; late endosome membrane; late endosome; early endosome; cytoplasm; plasma membrane; nucleolus; endosome membrane. Molecular Function: protein binding; ubiquitin binding; protein homodimerization activity; DNA binding; ubiquitin protein ligase binding; transcription corepressor activity; calcium-dependent protein binding. Biological Process: ubiquitin-dependent protein catabolic process via the multivesicular body pathway; viral reproduction; non-lytic virus budding; protein modification process; virus assembly; viral infectious cycle; endosome transport; keratinocyte differentiation; negative regulation of cell proliferation; protein transport; cell division; viral protein processing; regulation of cell growth; cell cycle arrest; negative regulation of transcription, DNA-dependent. Disease: Breast Cancer

0.002 mg

140 USD

0.01 mg

205

1 mg

5015