protein

Recombinant Human Tissue Inhibitor of Metalloprotease 1, HEK

MBS144066

0.01 mg

205 USD

Recombinant Protein

Recombinant Human Tissue Inhibitor of Metalloprotease 1, HEK

Tissue Inhibitor of Metalloprotease 1

TIMP1 Human, HEK; Tissue Inhibitor of Metalloprotease 1 Human Recombinant, HEK; Metalloproteinase inhibitor 1; Tissue inhibitor of metalloproteinases; TIMP-1; Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; TIMP1; CLGI; TIMP; EPO; HCI; FLJ90373; TIMP1 HEK

metalloproteinase inhibitor 1; Metalloproteinase inhibitor 1; metalloproteinase inhibitor 1; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1; TIMP metallopeptidase inhibitor 1; Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; TIMP-1

TIMP1

TIMP1; TIMP1; EPA; EPO; HCI; CLGI; TIMP; CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1

TIMP1_HUMAN

HEK293 Cells

207

Greater than 95.0% as determined by SDS-PAGE.

The TIMP1 protein was lyophilized after extensive dialysis against PBS. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized TIMP1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TIMP1 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized TIMP1 in sterile 18M?-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The IC50 of 2.5-4 nM is measured by its ability to inhibit recombinant human MMP-2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2.

ENZYMES; Enzymes; Metalloprotease

Description: TIMP1 Human Recombinant produced in HEK-293 cells is a secreted protein with the sequence of Human TIMP-1 (amino acids Cys24-Ala207) and fused to a polyhistidine tag at the C-terminus. Introduction: TIMP1 is a member of the TIMP family. TIMP1 is an inducible glycoprotein produced by various cell types. The TIMP1 glycoprotein is a natural inhibitor of the matrix metalloproteinases, which a group of peptidases involved in degradation of the extracellular matrix. TIMP1 binds in a reversible mode to MMPs, with regions in the N-terminal domain binding to the MMP substrate-binding site. On top of its inhibitory function against most of the known MMPs, TIMP1 is able to promote cell proliferation in a broad range of cell types, and may also have an anti-apoptotic role. Furthermore, TIMP1 has erthyroid-potentiating activity via translocation to the nucleus and also inhibits apoptosis in B-cells. The TIMP1 gene is situated within intron 6 of the synapsin I gene and is transcribed in the opposite direction. TIMP1 activity is dependent on the existence of disulfide bonds. TIMP1 transcription is extremely inducible in reaction to many cytokines and hormones. Increased TIMP1 levels are connecte

4507509

NP_003245.1

NM_003254.2

P01033

23,171 Da

Activation Of Matrix Metalloproteinases Pathway 576264!!Degradation Of The Extracellular Matrix Pathway 576263!!Extracellular Matrix Organization Pathway 576262!!HIF-1 Signaling Pathway 695200!!Hemostasis Pathway 106028!!IL6-mediated Signaling Events Pathway 137932!!Matrix Metalloproteinases Pathway 198900!!Platelet Activation, Signaling And Aggregation Pathway 106034!!Platelet Degranulation Pathway 106050!!Response To Elevated Platelet Cytosolic Ca2+ Pathway 106048

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]

0.01 mg

205 USD