protein

Recombinant Human Eukaryotic Translation Initiation Factor 2 Subunit 1 Alpha

MBS143979

0.01 mg

205 USD

Recombinant Protein

Recombinant Human Eukaryotic Translation Initiation Factor 2 Subunit 1 Alpha

Eukaryotic Translation Initiation Factor 2 Subunit 1 Alpha

EIF2S1 Human; Eukaryotic Translation Initiation Factor 2 Subunit 1 Alpha Human Recombinant; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; EIF-2alpha; EIF-2A; EIF2; EIF-2; EIF2A; EIF-2A

eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit 1; eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha

EIF2S1

EIF2S1; EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha

IF2A_HUMAN

E Coli

315

Greater than 85% as determined by SDS-PAGE.

0.5mg/ml solution containing 20mM Tris-HCl pH-8, 0.1M NaCl & 10% glycerol. Sterile filtered colorless solution.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Eukaryotic Translation Initiation Factor

Description: EIF2S1 Recombinant Human produced in E Coli is a single, non-glycosylated polypeptide chain containing 335 amino acids (1-315 a.a.) and having a molecular mass of 38.2 kDa. The EIF2S1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. Introduction: EIF2S1 participates in the premature steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA which binds to a 40S ribosomal subunit, followed by mRNA binding to create a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 should exchange with GTP by way of a reaction catalyzed by eIF-2B.

4758256

NP_004085.1

NM_004094.4

P05198

36,112 Da

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway 105970!!BDNF Signaling Pathway 712093!!Cap-dependent Translation Initiation Pathway 105967!!Eukaryotic Translation Initiation Pathway 105966!!Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway 105969!!GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway 105973!!Gene Expression Pathway 105937!!Hepatitis C Pathway 173973!!Hepatitis C Pathway 173907!!Herpes Simplex Infection Pathway 377873

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

0.01 mg

205 USD