protein

Recombinant Human Calreticulin

MBS143954

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Calreticulin

[Calreticulin]

[cC1qR; CRT; FLJ26680; RO; SSA; CRP55; Calreticulin; ERp60; CRTC; CALR]

[calreticulin; Calreticulin; calreticulin; CRP55; ERp60; HACBP; Sicca syndrome antigen A (autoantigen Ro; calreticulin); calregulin; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; grp60; calreticulin; CRP55; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP; grp60]

[CALR]

[CALR; CALR; RO; CRT; SSA; cC1qR; HEL-S-99n; CRTC; ERp60]

CALR_HUMAN

417

MGSSHHHHHH SSGLVPRGSH MEPAVYFKEQ FLDGDGWTSR WIESKHKSDF GKFVLSSGKF YGDEEKDKGL QTSQDARFYA LSASFEPFSN KGQTLVVQFT VKHEQNIDCG GGYVKLFPNS LDQTDMHGDS EYNIMFGPDI CGPGTKKVHV IFNYKGKNVL INKDIRCKDD EFTHLYTLIV RPDNTYEVKI DNSQVESGSL EDDWDFLPPK KIKDPDASKP EDWDERAKID DPTDSKPEDW DKPEHIPDPD AKKPEDWDEE MDGEWEPPVI QNPEYKGEWK PRQIDNPDYK GTWIHPEIDN PEYSPDPSIY AYDNFGVLGL DLWQVKSGTI FDNFLITNDE AYAEEFGNET WGVTKAAEKQ MKDKQDEEQR LKEEEEDKKR KEEEEAEDKE DDEDKDEDEE DEEDKEEDEE EDVPGQAKDE L

Greater than 85% as determined by SDS-PAGE.

1mg/ml solution containing 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl and 10% glycerol. Sterile filtered colorless solution.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Source: E Coli.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins

Description: CALR Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 421 amino acids (18-417 a.a.) and having a molecular mass of 48.7 kDa. CALR protein is fused to a 20 amino acid His-Tag at N-terminus and purified by standard chromatography. Introduction: CALR is a multifunctional protein that acts as a main Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin is localized in the nucleus, and participates in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is nearly identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. CALR binds to antibodies in specific sera of systemic lupus and Sjogren patients which have anti-Ro/SSA antibodies, it is well conserved among species, and it is positioned in the endoplasmic and sarcoplasmic reticulum where it binds calcium. The amino terminus of CALR interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. CALR reduces the binding of androgen receptor to its hormone-responsive DNA element and inhibits androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Therefore, CALR acts as a significant modulator of the regulation of gene transcription by nuclear hormone receptors.

4757900

NP_004334.1

NM_004343.3

P27797

ATF6-alpha Activates Chaperone Genes Pathway (530770); ATF6-alpha Activates Chaperones Pathway (105905); Adaptive Immune System Pathway (366160); Androgen Receptor Signaling Pathway (198806); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Asparagine N-linked Glycosylation Pathway (161013); Assembly Of Viral Components At The Budding Site Pathway (106084)

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq, Jul 2008]

Calreticulin: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57. Interacts with NR3C1 and TRIM21. Interacts with GABARAP. Belongs to the calreticulin family. Protein type: Secreted; Nuclear receptor co-regulator; Calcium-binding; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19p13.3-p13.2. Cellular Component: Golgi apparatus; extracellular space; cell surface; focal adhesion; smooth endoplasmic reticulum; sarcoplasmic reticulum lumen; endoplasmic reticulum; endoplasmic reticulum lumen; acrosome; extracellular region; cytosol; polysome; proteinaceous extracellular matrix; membrane; perinuclear region of cytoplasm; cytoplasm; intracellular; nucleus; external side of plasma membrane. Molecular Function: hormone binding; zinc ion binding; complement component C1q binding; unfolded protein binding; calcium ion binding; peptide binding; mRNA binding; integrin binding; protein binding; DNA binding; androgen receptor binding; iron ion binding; chaperone binding; ubiquitin protein ligase binding; carbohydrate binding; glycoprotein binding. Biological Process: protein maturation via protein folding; protein folding; regulation of meiosis; negative regulation of transcription from RNA polymerase II promoter; cardiac muscle cell differentiation; response to estradiol stimulus; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of transcription, DNA-dependent; positive regulation of phagocytosis; positive regulation of cell proliferation; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein export from nucleus; cortical actin cytoskeleton organization and biogenesis; cell cycle arrest; response to drug; receptor-mediated endocytosis; negative regulation of steroid hormone receptor signaling pathway; protein stabilization; unfolded protein response; positive regulation of cell cycle; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; response to testosterone stimulus; post-translational protein modification; peptide antigen assembly with MHC class I protein complex; cellular calcium ion homeostasis; negative regulation of retinoic acid receptor signaling pathway; negative regulation of neuron differentiation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; glucocorticoid receptor signaling pathway; negative regulation of translation; sequestering of calcium ion; protein amino acid N-linked glycosylation via asparagine; spermatogenesis; negative regulation of transcription, DNA-dependent; positive regulation of DNA replication. Disease: Myelofibrosis; Thrombocythemia 1

0.005 mg

140 USD

0.025 mg

205

1 mg

3515