protein

Recombinant Human O-6-Methylguanine-DNA Methyltransferase

MBS143892

0.005 mg

140 USD

Recombinant Protein

Recombinant Human O-6-Methylguanine-DNA Methyltransferase

O-6-Methylguanine-DNA Methyltransferase

MGMT Human; O-6-Methylguanine-DNA Methyltransferase Human Recombinant; Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; MGMT

PREDICTED: methylated-DNA--protein-cysteine methyltransferase isoform X1; Methylated-DNA--protein-cysteine methyltransferase; methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

MGMT

MGMT; MGMT; MGMT

MGMT_HUMAN

E Coli

207

Greater than 95.0% as determined by SDS-PAGE.

The MGMT solution contains 20mM Tris-HCl pH-7.5, 1mM DTT and 10% glycerol. Sterile Filtered clear colorless solution.

ENZYMES; Enzymes; Transferase

Description: MGMT Human Recombinant fused to a 20 amino acid His Tag at N-terminal produced in E Coli is a single, non-glycosylated, polypeptide chain containing 227 amino acids (1-207) and having a molecular mass of 23.8 kDa. The MGMT is purified by proprietary chromatographic techniques. Introduction: MGMT is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and takes part in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. abnormal MGMT expression correlates with the prognosis in human solid cancers. MGMT decrease of expression is correlated with methylation. The human MGMT is a negative regulator of estrogen receptor-mediated transcription upon alkylation DNA damage. MGMT promoter hypermethylation plays an important role in the early steps of colorectal carcinogenesis. Abnormal promoter hypermethylation of MGMT gene is associated with oral squamous cell carcinomas.

530394832

XP_005252739.1

XM_005252682.1

P16455

21,646 Da

DNA Damage Reversal Pathway (105857); DNA Repair Pathway (105837)

MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family. Protein type: Nuclear receptor co-regulator; EC 2.1.1.63; Apoptosis; DNA repair, damage; Methyltransferase. Chromosomal Location of Human Ortholog: 10q26. Cellular Component: nucleoplasm; membrane; nucleus. Molecular Function: methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; DNA binding; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity. Biological Process: response to drug; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to folic acid; response to toxin; DNA methylation; regulation of caspase activity; DNA repair; DNA ligation

0.005 mg

140 USD

0.02 mg

205

1 mg

3490