Recombinant Human LIN28 | MyBioSource MBS143880 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human LIN28

catalog :

MBS143880

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS143880

products type :

Recombinant Protein

products full name :

Recombinant Human LIN28

products short name :

LIN28

products name syn :

LIN28 Human; LIN28 Human Recombinant; CSDD1; FLJ12457; LIN-28; LIN28A; Protein lin-28 homolog A; ZCCHC1; Zinc finger CCHC domain-containing protein 1; Lin-28A; LIN28

other names :

protein lin-28 homolog A; Protein lin-28 homolog A; protein lin-28 homolog A; RNA-binding protein LIN-28; zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1; lin-28 homolog A (C. elegans); Zinc finger CCHC domain-containing protein 1

products gene name :

LIN28A

other gene names :

LIN28A; LIN28A; CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A; CSDD1; LIN28; ZCCHC1; Lin-28A

uniprot entry name :

LN28A_HUMAN

host :

E Coli

sequence length :

209

purity :

Greater than 90.0% as determined by SDS-PAGE

form :

The LIN28 protein solution contains 20mM Tris-HCl, pH-8, 10% glycerol and 0.1M NaCl. Sterile filtered colorless solution.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

products categories :

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; LIN Protein

products description :

Description: Recombinant Human LIN28 produced in E Coli is a single, non-glycosylated polypeptide chain containing 191 amino acids (42-209) and having a molecular mass of 21.1 kDa.LIN28 is expressed with a 23 amino acid His tag fused at N-Terminus and purified by proprietary chromatographic techniques. Introduction: LIN28 plays an important role as a 'translational enhancer', leading specific mRNAs to polysomes and therefore increasing the competence of protein synthesis. LIN28 is a marker of undifferentiated human embryonic stem cells and it enhances the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. LIN28 binds to the let-7 pre-miRNA and blocks production of the mature let-7 microRNA in mouse embryonic stem cells. Overexpression of LIN28 is associated with human germ-cell tumors.

ncbi gi num :

13375938

ncbi acc num :

NP_078950.1

ncbi gb acc num :

NM_024674.4

uniprot acc num :

Q9H9Z2

ncbi mol weight :

22,743 Da

ncbi pathways :

Cardiac Progenitor Differentiation Pathway (712094); Developmental Biology Pathway (477129); Transcriptional Regulation Of Pluripotent Stem Cells Pathway (1127696)

uniprot summary :

LIN28A: Acts as a translational enhancer , driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5 -GGAG-3 motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5 -GGAG-3 motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Monomer. During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent. Interacts with ZCCHC11/TUT4. Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3 -UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid. Expressed in embryonic stem cells (ES cells), placenta and testis. Belongs to the lin-28 family. Protein type: Translation; RNA-binding; Nucleolus. Chromosomal Location of Human Ortholog: 1p36.11. Cellular Component: stress granule; cytoplasm; nucleolus; nucleus; cytosol. Molecular Function: mRNA binding; protein binding; miRNA binding; DNA binding; zinc ion binding; RNA binding; translation initiation factor binding. Biological Process: positive regulation of translation; regulation of transcription, DNA-dependent; pre-microRNA processing; somatic stem cell maintenance; stem cell maintenance; positive regulation of neuron differentiation; RNA 3 -end processing; germ cell development; negative regulation of glial cell differentiation

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

5230

more info or order :

MyBioSource product webpage