Recombinant Human Aldehyde Dehydrogenase 2 | MyBioSource MBS143867 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Aldehyde Dehydrogenase 2

catalog :

MBS143867

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS143867

products type :

Recombinant Protein

products full name :

Recombinant Human Aldehyde Dehydrogenase 2

products short name :

Aldehyde Dehydrogenase 2

products name syn :

ALDH2 Human; Aldehyde Dehydrogenase Human Recombinant; ALDM; ALDHI; ALDH-E2; MGC1806; ALDH2; Aldehyde dehydrogenase mitochondrial; ALDH class 2

other names :

aldehyde dehydrogenase, mitochondrial isoform 1; Aldehyde dehydrogenase, mitochondrial; aldehyde dehydrogenase, mitochondrial; ALDH class 2; acetaldehyde dehydrogenase 2; liver mitochondrial ALDH; nucleus-encoded mitochondrial aldehyde dehydrogenase 2; aldehyde dehydrogenase 2 family (mitochondrial); ALDH class 2; ALDH-E2; ALDHI

products gene name :

ALDH2

other gene names :

ALDH2; ALDH2; ALDM; ALDHI; ALDH-E2; ALDM

uniprot entry name :

ALDH2_HUMAN

host :

E Coli

sequence length :

517

sequence :

MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLNRLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAGTVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S.

purity :

Greater than 90.0% as determined by SDS-PAGE.

form :

ALDH2 protein (1mg/ml) contains 20mM Tris-HCl buffer, pH-7.5, 1mM DTT, 1mM EDTA and 10% Glycerol. Sterile Filtered clear solution.

concentration :

1mg/ml

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Biological Activity: Specific activity was found to be > 0.14 units/ml. Activity was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. 1 unit will oxidize 1umole of acetaldehyde to acetic acid per minute at pH 8 at 25C in the presence of beta-NAD, potassium and thiols.

products categories :

ENZYMES; Enzymes; Dehydrogenase

products description :

Description: ALDH2 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 501 amino acids (18-517 a.a.) & having a molecular mass of 54.5 kDa. The ALDH2 is purified by proprietary chromatographic techniques. Introduction: ALDH2 is part of the aldehyde dehydrogenase family of proteins which catalyze the chemical transformation from acetaldehyde to acetic acid. ALDH2 is the second enzyme of the major oxidative pathway of alcohol metabolism. ALDH2 has 2 major liver isoforms: cytosolic and mitochondrial, which differ by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Nearly all Caucasians have 2 major isozymes, whereas roughly 50% of Orientals have only the cytosolic isozyme, omitting the mitochondrial isozyme. The extremely higher rate of acute alcohol intoxication with Orientals compared to Caucasians is due to the fact of the absence of mitochondrial isozyme. ALDH2 has a low Km for acetaldehydes, and is localized in mitochondrial matrix.

ncbi gi num :

25777732

ncbi acc num :

NP_000681.2

ncbi gb acc num :

NM_000690.3

uniprot acc num :

P05091

ncbi mol weight :

50,989 Da

ncbi pathways :

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Ascorbate And Aldarate Metabolism Pathway (82932); Ascorbate And Aldarate Metabolism Pathway (293); Biological Oxidations Pathway (105698); Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway (1127640); Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway (1127641); Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway (1127642); Defective CYP17A1 Causes Adrenal Hyperplasia 5 (AH5) Pathway (1127643); Defective CYP19A1 Causes Aromatase Excess Syndrome (AEXS) Pathway (1127644)

ncbi summary :

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]

uniprot summary :

ALDH2: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]. Protein type: Amino Acid Metabolism - lysine degradation; Amino Acid Metabolism - arginine and proline; Oxidoreductase; Amino Acid Metabolism - tryptophan; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - butanoate; EC 1.2.1.3; Amino Acid Metabolism - histidine; Mitochondrial; Lipid Metabolism - glycerolipid; Lipid Metabolism - fatty acid; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - ascorbate and aldarate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Secondary Metabolites Metabolism - limonene and pinene degradation; Other Amino Acids Metabolism - beta-alanine. Chromosomal Location of Human Ortholog: 12q24.2. Cellular Component: mitochondrial matrix. Molecular Function: aldehyde dehydrogenase (NAD) activity; electron carrier activity; aldehyde dehydrogenase [NAD(P)+] activity. Biological Process: synaptic transmission; ethanol catabolic process; xenobiotic metabolic process; alcohol metabolic process; carbohydrate metabolic process; ethanol oxidation; neurotransmitter biosynthetic process. Disease: Alcohol Sensitivity, Acute

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

205

size3 :

1 mg

price3 :

1790

more info or order :

MyBioSource product webpage