Recombinant Human Non-Metastatic Cells 2 | MyBioSource MBS143797 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Non-Metastatic Cells 2

catalog :

MBS143797

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS143797

products type :

Recombinant Protein

products full name :

Recombinant Human Non-Metastatic Cells 2

products short name :

Non-Metastatic Cells 2

products name syn :

NME2 Human; Non-Metastatic Cells 2 Human Recombinant; Nucleoside diphosphate kinase B; NDPK-B; NDPKB; NM23-H2; NM23B; EC 2.7.4.6; NDP kinase B; C-myc purine-binding transcription factor PUF; NDK B; NME2; puf; MGC111212

other names :

NME1-NME2 protein; Nucleoside diphosphate kinase B; NME1-NME2 protein; NME1-NME2 readthrough transcript; NME1-NME2 readthrough; C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB (EC:2.7.13.3); nm23-H2

products gene name :

NME2

other gene names :

NME1-NME2; NME2; NMELV; NM23-LV; NM23B; NDK B; NDP kinase B

uniprot entry name :

NDKB_HUMAN

host :

E Coli

sequence length :

267

sequence :

MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE.

purity :

Greater than 95.0% as determined by SDS-PAGE.

form :

The NME2 protein solution contains 20mM Tris-HCl pH-8, 1mM DTT, and 10% glycerol. Sterile Filtered colorless solution.

storage stability :

NME2 although stable 4 degree C for 4 weeks, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

products categories :

PROTEIN KINASES; Recombinant Proteins; Non-Metastatic Cells

products description :

Description: NME2 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (1-152 a.a.) and having a molecular mass of 17.2kDa. The NME2 is purified by conventional chromatography. Introduction: NME2 takes an important part in the synthesis of nucleoside triphosphates other than ATP. NME2 negatively controls Rho activity by interacting with AKAP13/LBC. NME2 acts as a transcriptional activator of the MYC gene. NME2 binds DNA non-specifically. NME2 is a heterodimeric enzyme functioning as a nucleoside diphosphate kinase. NME1 and NME2 contain 152 amino acids, A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases participate in the synthesis of nucleoside triphosphates, and NM23 is involved in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways.

ncbi gi num :

66392203

ncbi acc num :

NP_001018146.1

ncbi gb acc num :

NM_001018136.2

uniprot acc num :

P22392

ncbi mol weight :

30,137 Da

ncbi pathways :

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (618580); Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (468242); CMP Phosphorylation Pathway (782393); CMP Phosphorylation Pathway (908070); Guanine Ribonucleotide Biosynthesis IMP = GDP,GTP Pathway (618581); Guanine Ribonucleotide Biosynthesis IMP = GDP,GTP Pathway (468243); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Nucleotide Metabolism Pathway (198876)

ncbi summary :

This locus represents naturally occurring read-through transcription between the neighboring NME1 and NME2 genes. The significance of this read-through transcription and the function of the resulting protein product have not yet been determined. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2010]

uniprot summary :

NME2: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Nucleotide Metabolism - pyrimidine; Kinase, nucleoside diphosphate; Nuclear receptor co-regulator; EC 2.7.13.3; Kinase, other; Nucleotide Metabolism - purine; EC 2.7.4.6; Other group; NDK family. Chromosomal Location of Human Ortholog: 17q21.3. Cellular Component: ruffle; centrosome; focal adhesion; mitochondrion; membrane; perinuclear region of cytoplasm; lamellipodium; cytoplasm; intermediate filament; nucleus; cytosol. Molecular Function: protein binding; gamma-tubulin binding; metal ion binding; protein histidine kinase activity; intermediate filament binding; nucleoside diphosphate kinase activity; transcription factor activity; protein kinase binding; ATP binding; single-stranded DNA binding. Biological Process: GTP biosynthetic process; integrin-mediated signaling pathway; peptidyl-histidine phosphorylation; response to cAMP; transcription, DNA-dependent; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of myeloid leukocyte differentiation; UTP biosynthetic process; hippocampus development; nucleoside diphosphate phosphorylation; response to testosterone stimulus; CTP biosynthetic process; regulation of transcription, DNA-dependent; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; cell adhesion; positive regulation of epithelial cell proliferation; regulation of epidermis development; response to amine stimulus; positive regulation of keratinocyte differentiation; purine nucleotide metabolic process; negative regulation of apoptosis

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.025 mg

price2 :

205

size3 :

1 mg

price3 :

2220

more info or order :

MyBioSource product webpage