protein

Recombinant Human Transferrin

MBS143791

100 mg

140 USD

Recombinant Protein

Recombinant Human Transferrin

Transferrin

Transferrin Human; Transferrin Human Recombinant; Serotransferrin; Transferrin; Siderophilin; Beta-1-metal-binding globulin; TF; PRO1557; PRO2086; DKFZp781D0156; HTF; Transferrin

serotransferrin; Serotransferrin; serotransferrin; beta-1 metal-binding globulin; siderophilin; transferrin; Beta-1 metal-binding globulin; Siderophilin

TF; TF; TFQTL1; PRO1557; PRO2086; Transferrin

TRFE_HUMAN

Oryza sativa (rice)

698

Purity as determined by SDS-PAGE is 95%.

The protein (1mg/ml) was lyophilized with no additives. Sterile Filtered lyophilized (freeze-dried) powder.

1mg/ml

Lyophilized Transferrin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Transferrin Human Recombinant should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Recombinant Human Transferrin has increased the growth of numerous cell types including hybridoma and primary cell lines. The optimal concentration of Recombinant Human Transferrin for cell culture differs with the cell line. In CHO, effective concentrations range from 10-50 mg/liter. The reported effective concentrations for hybridoma and Vero cell lines range from 5mg/liter to 20mg/liter. Effective concentrations for primary cells range between 5mg/liter to 100mg/liter.

Advantages: Animal component-free. Well defined and consistent. Universal iron carrier in cell culture.Replaces plasma-derived human and bovine transferrin. Replaces iron chelators. Iron: The average amount of Ferric Iron is 0.4-0.5 ug per mg of protein (protein concentration is measured by absorption at 280 nm).

Solubility: Stock solutions can be prepared by dissolving gently into PBS for several minutes. Recommended stock concentrations are 5mg/ml to 20 mg/ml in PBS, though others can be used as well. Please try to avoid the formation of bubbles when dissolving the protein. Sterile filter through 0.2 um filter. Activity: One mg of Recombinant Human Transferrin will bind to approximately 2 micrograms of Fe.

RECOMBINANT & NATURAL PROTEINS; Natural Proteins; Transferrin

Description: Recombinant Human Transferrin produced in Plant is a non-glycosylated, polypeptide chain containing 679 amino acids and having a molecular mass of 76 kDa. The Recombinant Human Transferrin is purified by proprietary chromatographic techniques. Introduction: Transferrin is the iron-transport protein of vertebrate serum and donates iron to cells through interaction with a specific membrane receptor, CD71. Transferrin appears to be indispensable for most cells growing in tissue culture. It is referred to frequently as a growth factor because, in analogy to other growth factor-receptor interactions, proliferating cells express high numbers of transferrin receptors, and the binding of transferrin to their receptors is needed for cells to initiate and maintain their DNA synthesis. Apart from its role as an iron transport protein transferrin acts as a cytokine and has functions that may not be related to its iron-carrying capacity.

4557871

NP_001054.1

NM_001063.3

P02787

77,064 Da

EPHB Forward Signaling Pathway (138047); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); Hemostasis Pathway (106028); Iron Metabolism In Placenta Pathway (672461); Iron Uptake And Transport Pathway (187191); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050)

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q22.1. Cellular Component: extracellular space; cell surface; early endosome; cytoplasmic membrane-bound vesicle; extracellular region; coated pit; recycling endosome; endocytic vesicle; perinuclear region of cytoplasm; apical plasma membrane; late endosome; basal plasma membrane; endosome membrane; vesicle. Molecular Function: protein binding; ferric iron binding; ubiquitin protein ligase binding. Biological Process: platelet activation; retinal homeostasis; platelet degranulation; cellular iron ion homeostasis; transferrin transport; blood coagulation; transmembrane transport. Disease: Atransferrinemia

100 mg

140 USD

1 g

410

10 g

2920