Recombinant Human Glucose-6-Phosphate Isomerase | MyBioSource MBS143757 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Glucose-6-Phosphate Isomerase

catalog :

MBS143757

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS143757

products type :

Recombinant Protein

products full name :

Recombinant Human Glucose-6-Phosphate Isomerase

products short name :

Glucose-6-Phosphate Isomerase

products name syn :

GPI Human; Glucose-6-Phosphate Isomerase Human Recombinant; Glucose-6-phosphate isomerase; Phosphoglucose isomerase; Phosphohexose isomerase; Autocrine motility factor; Neuroleukin; Sperm antigen 36; GPI; PGI; PHI; AMF; NLK; SA-36; GNPI

other names :

glucose-6-phosphate isomerase isoform 2; Glucose-6-phosphate isomerase; glucose-6-phosphate isomerase; autocrine motility factor; glucose phosphate isomerase; hexose monophosphate isomerase; hexosephosphate isomerase; neuroleukin; oxoisomerase; phosphoglucose isomerase; phosphohexomutase; phosphohexose isomerase; phosphosaccharomutase; sperm antigen 36; sperm antigen-36; glucose-6-phosphate isomerase; Autocrine motility factor; AMF; Neuroleukin; NLK; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI; Sperm antigen 36; SA-36

products gene name :

GPI

other gene names :

GPI; GPI; AMF; NLK; PGI; PHI; GNPI; SA36; SA-36; GPI; AMF; NLK; PGI; PHI; SA-36

uniprot entry name :

G6PI_HUMAN

host :

E Coli

sequence length :

558

sequence :

MGSSHHHHHH SSGLVPRGSH MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ.

purity :

Greater than 95.0% as determined by SDS-PAGE.

form :

The GPI solution contains 20mM Tris-HCl buffer (pH8.0), 1mM DTT and 10% glycerol. Sterile Filtered colorless solution.

concentration :

1mg/ml

storage stability :

GPI although stable 4 degree C for 4 weeks, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

products categories :

ENZYMES; Enzymes; Isomerase

products description :

Description: GPI Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 578 amino acids (1-558 a.a.) and having a molecular mass of 65.3kDa.The GPI is purified by proprietary chromatographic techniques. Introduction: Glucose-6-phosphate isomerase (GPI) is a part of the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. GPI is a dimeric enzyme which catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Mammalian GPI also functions as a tumor-secreted cytokine and an angiogenic factor (AMF) which stimulates endothelial cell motility. In addition, GPI is a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. GPI performs in different capacities inside and outside the cell. In the cytoplasm, GPI is involved in glycolysis and gluconeogenesis, while outside the cell it acts as a neurotrophic factor for spinal and sensory neurons. Defects in the GPI gene cause the nonspherocytic hemolytic anemia and a severe enzyme deficiency can be linked to hydrops fetalis, immediate neonatal death and neurological impairment.

ncbi gi num :

18201905

ncbi acc num :

NP_000166.2

ncbi gb acc num :

NM_000175.3

uniprot acc num :

P06744

ncbi mol weight :

64,325 Da

ncbi pathways :

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); Gluconeogenesis Pathway (106204); Glucose Metabolism Pathway (106199); Glycogen Storage Diseases Pathway (1127581); Glycolysis Pathway (105911); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (413340)

ncbi summary :

This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

uniprot summary :

G6PI: belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - pentose phosphate pathway; Apoptosis; Cytokine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase; EC 5.3.1.9. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: nucleoplasm; extracellular space; neuron projection; membrane; cytoplasm; plasma membrane; cytosol. Molecular Function: monosaccharide binding; glucose-6-phosphate isomerase activity; growth factor activity; cytokine activity; intramolecular transferase activity. Biological Process: aldehyde catabolic process; methylglyoxal biosynthetic process; glycolysis; learning and/or memory; hemostasis; carbohydrate metabolic process; glucose 6-phosphate metabolic process; glucose metabolic process; pathogenesis; angiogenesis; negative regulation of caspase activity; negative regulation of neuron apoptosis; humoral immune response; gluconeogenesis. Disease: Hemolytic Anemia, Nonspherocytic, Due To Glucose Phosphate Isomerase Deficiency

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

2665

more info or order :

MyBioSource product webpage