product summary
Loading...
company name :
MyBioSource
product type :
protein
product name :
Recombinant Pufferfish Leptin
catalog :
MBS143749
quantity :
0.02 mg
price :
140 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS143749
products type :
Recombinant Protein
products full name :
Recombinant Pufferfish Leptin
products short name :
Pufferfish Leptin
products name syn :
Leptin Pufferfish; Leptin Pufferfish Recombinant; OB Protein; Obesity Protein; OBS; Obesity factor
other names :
leptin; Leptin; leptin
products gene name :
pfLeptin
other gene names :
lep; lep
uniprot entry name :
LEP_TAKRU
host :
E Coli
sequence length :
152
sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Pro-Gly-Ala.
purity :
Greater than 99.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
form :
The Pufferfish Leptin was lyophilized from a concentrated (0.85mg/ml) solution with 0.003mM NaHCO3. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Pufferfish Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Leptin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Pufferfish Leptin in sterile 0.4% NaHCO3 pH-9 not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 1.28 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Biological Activity: Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. The affinity of human leptin receptors is considerably lower campared to mammalian leptins.
products categories :
CYTOKINES AND GROWTH FACTORS; Growth Factors; Leptin
products description :
Description: Leptin Pufferfish (Takifugu rubripes) Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 16 kDa. Bioactive Leptin Pufferfish (Takifugu rubripes) Recombinant was prepared according to the sequence published by Kurokawa et al. (2005)Peptides 26, 745-750 in two forms: monomer and covalent dimer. MS analysis revealed molecular masses of 15,291 and 30,585 Da, close to the theoretical values of 15,270 and 30,540 Da. CD spectra revealed high similarity to mammalian leptins. Other details of its preparation will be soon published by Yacobovitz et al (in press), General and Comparative Endocrinology.The Pufferfish Leptin is purified by proprietary chromatographic techniques. Introduction: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.
ncbi gi num :
74096427
ncbi acc num :
NP_001027897.1
ncbi gb acc num :
NM_001032725.1
uniprot acc num :
Q588G0
ncbi mol weight :
17,204 Da
size1 :
0.02 mg
price1 :
140 USD
size2 :
0.1 mg
price2 :
205
size3 :
1 mg
price3 :
1075
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!