protein

Recombinant Human Thymus Expressed Chemokine (CCL25)

MBS143721

0.02 mg

205 USD

Recombinant Protein

Recombinant Human Thymus Expressed Chemokine (CCL25)

Thymus Expressed Chemokine

TECK Human; Thymus Expressed Chemokine Human Recombinant (CCL25); C-C motif chemokine 25; Small-inducible cytokine A25; Thymus-expressed chemokine; Chemokine TECK; CCL25; SCYA25; TECK; Ckb15; MGC150327

C-C motif chemokine 25 isoform 2; C-C motif chemokine 25; C-C motif chemokine 25; Ck beta-15; TECKvar; chemokine TECK; small inducible cytokine subfamily A (Cys-Cys), member 25; small-inducible cytokine A25; thymus expressed chemokine; thymus-expressed chemokine; chemokine (C-C motif) ligand 25; Chemokine TECK; Small-inducible cytokine A25; Thymus-expressed chemokine

TECK

CCL25; CCL25; TECK; Ckb15; SCYA25; SCYA25; TECK

CCL25_HUMAN

E Coli

149

QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSRKNVSLL ISANSGL.

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Filtered (0.2 um) and lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized TECK although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TECK should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized TECK in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: Determined by its ability to chemoattract human monocytes using a concentration range of 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.

CHEMOKINES; Chemokines

Description: TECK Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14.2kDa. The TECK is purified by proprietary chromatographic techniques. Introduction: CCL25 (Teck) is a novel CC chemokine, which is distantly related (about 20% amino acid sequence identity) to other CC chemokines. The mouse CCL25 cDNA has also been cloned and shown to encode a 144 a.a. protein, which exhibits 49% a.a. sequence identity to the human CCL25. Human and mouse CCL25 expression was shown to be greatly restricted to the thymus and small intestine. While dendritic cells are identified as the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. CCL25 signals through the CCR9 receptor. Teck is possibly involved in T-cell development. Recombinant human and mouse Teck were shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. The recombinant protein demonstrates chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritic cells but is inactive on peripheral blood lymphocytes and neutrophils.

323098324

NP_001188288.1

NM_001201359.1

O15444

16,538 Da

Chemokine Receptors Bind Chemokines Pathway 106359!!Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway 1127664!!Disease Pathway 530764!!G Alpha (i) Signalling Events Pathway 119550!!GPCR Downstream Signaling Pathway 119548

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. The product of this gene binds to chemokine receptor CCR9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]

0.02 mg

205 USD