Recombinant Human NAD(P)H Dehydrogenase Quinone 1 | MyBioSource MBS143702 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human NAD(P)H Dehydrogenase Quinone 1

catalog :

MBS143702

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS143702

products type :

Recombinant Protein

products full name :

Recombinant Human NAD(P)H Dehydrogenase Quinone 1

products short name :

NAD(P)H Dehydrogenase Quinone 1

products name syn :

NQO1 Human; NAD(P)H Dehydrogenase Quinone 1 Human Recombinant; NAD(P)H dehydrogenase (quinone) 1; Quinone reductase 1; QR1; NAD(P)H:quinone oxidoreductase 1; DT-diaphorase; DTD; Azoreductase; Phylloquinone reductase; Menadione reductase; NQO1; DIA4; NMOR1; DHQU; NMORI

other names :

NAD(P)H dehydrogenase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase [quinone] 1; DT-diaphorase; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; azoreductase; diaphorase (NADH/NADPH) (cytochrome b-5 reductase); diaphorase-4; dioxin-inducible 1; menadione reductase; phylloquinone reductase; quinone reductase 1; NAD(P)H dehydrogenase, quinone 1; Azoreductase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1; QR1

products gene name :

NQO1

other gene names :

NQO1; NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; DIA4; NMOR1; DTD; QR1

uniprot entry name :

NQO1_HUMAN

host :

E Coli

sequence length :

274

sequence :

MGSSHHHHHH SSGLVPRGSH MVGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK.

purity :

Greater than 95.0% as determined by SDS-PAGE.

form :

The NQO1 solution contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1mM DTT. Sterile Filtered colorless solution.

concentration :

1 mg/ml

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

products categories :

ENZYMES; Enzymes; Dehydrogenase

products description :

Description: NQO1 Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 294 amino acids (1-274 a.a.) and having a molecular mass of 33kDa.The NQO1 is purified by proprietary chromatographic techniques. Introduction: NQO1 belongs to the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. NQO1 acts as an imperative part of cellular antioxidant defense by detoxifying quinines therefore preventing the formation of reactive oxygen species. It seems that NQO1 serves as a quinone reductase relating to conjugation reactions of hydroquinons involved in detoxification pathways in addition to biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Altered NQO1 expression is seen in many tumors and also linked to Alzheimer's disease. NQO1 gene mutations are linked to tardive dyskinesia which is an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer.

ncbi gi num :

4505415

ncbi acc num :

NP_000894.1

ncbi gb acc num :

NM_000903.2

uniprot acc num :

P15559

ncbi mol weight :

26,365 Da

ncbi pathways :

AhR Pathway (755436); Dopamine Metabolism Pathway (920995); Estrogen Metabolism Pathway (198898); Keap1-Nrf2 Pathway (198777); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Oxidative Stress Pathway (198916); Regulation Of Ornithine Decarboxylase (ODC) Pathway (106176); Ubiquinone And Other Terpenoid-quinone Biosynthesis Pathway (82939); Ubiquinone And Other Terpenoid-quinone Biosynthesis Pathway (300)

ncbi summary :

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

NQO1: a FAD-binding flavoprotein enzyme that that prevents the one electron reduction of quinones that results in the production of radical species. Involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Mutations have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues. Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development. A homozygous common missense variant (NQO1(*)2, rs1800566(T)), that disables NQO1 strongly predicts poor survival among two independent series of women with breast cancer. Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turn leads to myeloid hyperplasia of bone marrow. Altered expression is associated with Alzheimer s disease (AD). Inhibited by dicoumarol. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 1.6.5.2; Oxidoreductase. Chromosomal Location of Human Ortholog: 16q22.1. Cellular Component: cell soma; cytoplasm; cytosol. Molecular Function: identical protein binding; protein binding; NAD(P)H dehydrogenase (quinone) activity; cytochrome-b5 reductase activity; superoxide dismutase activity. Biological Process: response to ethanol; removal of superoxide radicals; positive regulation of neuron apoptosis; xenobiotic metabolic process; response to toxin; negative regulation of catalytic activity; nitric oxide biosynthetic process; regulation of amino acid metabolic process; response to estradiol stimulus; response to nutrient; synaptic transmission, cholinergic; aging

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

5015

more info or order :

MyBioSource product webpage