Recombinant Human Poly (ADP-Ribose) Polymerase 1 | MyBioSource MBS143690 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Poly (ADP-Ribose) Polymerase 1

catalog :

MBS143690

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS143690

products type :

Recombinant Protein

products full name :

Recombinant Human Poly (ADP-Ribose) Polymerase 1

products short name :

Polymerase 1

products name syn :

PARP1 Human; Poly (ADP-Ribose) Polymerase 1 Human Recombinant; ADPRT; ADPRT1; pADPRT; pADPRT-1; PARP; PARP-1; PPOL; Poly [ADP-ribose] polymerase 1; NAD(+) ADP-ribosyltransferase 1; Poly[ADP-ribose] synthase 1; PARP1

other names :

poly; Poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase NAD(+); ADP-ribosyltransferase diphtheria toxin-like 1; NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; poly (ADP-ribose) polymerase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1

products gene name :

PARP1

other gene names :

PARP1; PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1

uniprot entry name :

PARP1_HUMAN

host :

E Coli

sequence length :

1014

sequence :

MKSKLPKPVQ DLIKMIFDVE SMKKAMVEYE IDLQKMPLGK LSKRQIQAAY SILSEVQQAV SQGSSDSQIL DLSNRFYTLI PHDFGMKKPP LLNNADSVQA KAEMLDNLLD IEVAYSLLRG GSDDSSKDPI DVNYEKLKTD IKVVDRDSEE AEIIRKYVKN THATTHNAYD LEVIDIFKIE REGECQRYKP FKQLHNRRLL WHGSRTTNFA GILSQGLRIA PPEAPVTGYM FGKGIYFADM VSKSANYCHT SQGDPIGLIL LGEVALGNMY ELKHASHISK LPKGKHSVKG LGKTTPDPSA NISLDGVDVP LGTGISSGVN DTSLLYNEYI VYDIAQVNLK YLLKLKFNFK TSLW.

purity :

Greater than 95% as determined by SDS-PAGE.

form :

PARP1 solution containing 20mM Tris pH-8, 1Mm DTT and 10% glycerol. Sterile filtered colorless solution.

storage stability :

PARP1 Human Recombinant although stable at 4 degree C for 1 week, should be stored below -18 degree C. Please prevent freeze thaw cycles.

products categories :

ENZYMES; Enzymes; Polymerase

products description :

Description: PARP1 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 354 amino acids (662-1014a.a.) and having a molecular mass of 39.6 kDa. PARP1 is purified by proprietary chromatographic techniques. Introduction: PARP1 takes part in the base excision repair pathway, by catalyzing the poly ADP-ribosyl of a restricted number of acceptor proteins involved in chromatin architecture and in DNA metabolism. PARP1 mediates the poly ADP-ribosy of APLF and CHFR. PARP1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. PARP1 is a chromatin-associated enzyme, poly (ADP-ribosyl) transferase, which modifies various nuclear proteins by poly ADP-ribosyl. PARP1 takes part in the regulation of various significant cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. PARP1 is a site of mutation in Fanconi anemia, and is involved in the pathophysiology of type I diabetes.

ncbi gi num :

156523968

ncbi acc num :

NP_001609.2

ncbi gb acc num :

NM_001618.3

uniprot acc num :

P09874

ncbi mol weight :

113,084 Da

ncbi pathways :

BER Complex Pathway (413429); BER Complex Pathway (468377); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Caspase Cascade In Apoptosis Pathway (137974); Corticotropin-releasing Hormone Pathway (920957); Disease Pathway (530764); Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway (645266); FAS Pathway And Stress Induction Of HSP Regulation (198894); Gene Expression Pathway (105937)

ncbi summary :

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

uniprot summary :

PARP1: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK. Protein type: Nuclear receptor co-regulator; Nucleolus; EC 2.4.2.30; DNA repair, damage; Nuclear envelope; Transferase. Chromosomal Location of Human Ortholog: 1q41-q42. Cellular Component: nucleoplasm; transcription factor complex; membrane; nucleolus; nuclear envelope; nucleus. Molecular Function: identical protein binding; protein binding; enzyme binding; DNA binding; zinc ion binding; protein N-terminus binding; NAD binding; transcription factor binding; protein kinase binding; NAD+ ADP-ribosyltransferase activity. Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; macrophage differentiation; transcription, DNA-dependent; DNA damage response, detection of DNA damage; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein autoprocessing; protein amino acid ADP-ribosylation; cellular response to insulin stimulus; base-excision repair; double-strand break repair; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; regulation of growth rate; telomere maintenance

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.025 mg

price2 :

205

size3 :

1 mg

price3 :

2220

more info or order :

MyBioSource product webpage