Recombinant Human Aldose Reductase | MyBioSource MBS143685 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Aldose Reductase

catalog :

MBS143685

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS143685

products type :

Recombinant Protein

products full name :

Recombinant Human Aldose Reductase

products short name :

Aldose Reductase

products name syn :

AKR1B1 Human; Aldose Reductase Human Recombinant; Aldehyde Reductase; EC 1.1.1.21; ALR2; ALDR1; MGC1804; Aldo-keto reductase family1 member B1; Aldose Reductase; AKR1B1; AR; ADR

other names :

aldose reductase; Aldose reductase; aldose reductase; Lii5-2 CTCL tumor antigen; aldehyde reductase 1; aldo-keto reductase family 1 member B1; low Km aldose reductase; aldo-keto reductase family 1, member B1 (aldose reductase); Aldehyde reductase; Aldo-keto reductase family 1 member B1

products gene name :

AKR1B1

other gene names :

AKR1B1; AKR1B1; AR; ADR; ALR2; ALDR1; ALDR1; AR

uniprot entry name :

ALDR_HUMAN

host :

E Coli

sequence length :

316

sequence :

MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF.

purity :

Greater than 95.0% as determined by SDS-PAGE.

form :

The 1mg/ml protein solution contains 20mM Tris-HCl buffer pH 8, 10% glycerol, and 1mM DTT. Sterile filtered colorless solution.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

other info2 :

Biological Activity: Specific activity : approximately 0.2 - 0.9 units/mg.Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25 degree C. Specific activity was expressed as units/mg protein.

products categories :

ENZYMES; Enzymes; Reductase

products description :

Description: AKR1B1 Human Recombinant amino produced in E Coli is a single, non-glycosylated polypeptide chain containing 316 amino acids having a molecular mass of 35.8 kDa.The AKR1B1 is purified by proprietary chromatographic techniques. Introduction: AKR1B1 is part of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. AKR1B1 catalyzes the reduction several aldehydes, including the aldehyde form of glucose, and thus involved in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. AKR1B1 catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Transgenic mice over expressing human aldose reductase show that AKR1B1 is a key player in ischemic injury and impairment of functional and metabolic recovery after ischemia. Aldose Reductase is an obligatory mediator of TNF-alpha signaling leading to an increase in the expression of adhesion molecules and increased binding of monocytes to the endothelium. AKR1B1 is a critical regulator of TNF-alpha-induced apoptotic signaling in endothelial cells.

ncbi gi num :

4502049

ncbi acc num :

NP_001619.1

ncbi gb acc num :

NM_001628.2

uniprot acc num :

P15121

ncbi mol weight :

35,853 Da

ncbi pathways :

Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Galactose Metabolism Pathway (82931); Galactose Metabolism Pathway (292); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150)

ncbi summary :

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]

uniprot summary :

AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family. Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Oxidoreductase; EC 1.1.1.21; Carbohydrate Metabolism - pyruvate; Lipid Metabolism - glycerolipid; Carbohydrate Metabolism - pentose and glucuronate interconversions. Chromosomal Location of Human Ortholog: 7q35. Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol. Molecular Function: glyceraldehyde oxidoreductase activity; electron carrier activity; aldo-keto reductase activity; aldehyde reductase activity. Biological Process: steroid metabolic process; carbohydrate metabolic process; C21-steroid hormone biosynthetic process; response to stress

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

205

size3 :

1 mg

price3 :

1790

more info or order :

MyBioSource product webpage