Recombinant Human Glycerol-3-Phosphate Dehydrogenase 2 | MyBioSource MBS143667 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Glycerol-3-Phosphate Dehydrogenase 2

catalog :

MBS143667

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS143667

products type :

Recombinant Protein

products full name :

Recombinant Human Glycerol-3-Phosphate Dehydrogenase 2

products short name :

Glycerol-3-Phosphate Dehydrogenase 2

products name syn :

GPD2 Human; Glycerol-3-Phosphate Dehydrogenase 2 Human Recombinant; Glycerol-3-phosphate dehydrogenase mitochondrial; glycerol-3-phosphate dehydrogenase 2 (mitochondrial); GPDH-M; GPD-M; mtGPD; GPD2; GDH2; GPDM; mGPDH

other names :

glycerol-3-phosphate dehydrogenase, mitochondrial; Glycerol-3-phosphate dehydrogenase, mitochondrial; glycerol-3-phosphate dehydrogenase, mitochondrial; GPD-M; GPDH-M; mitochondrial glycerophosphate dehydrogenase; mtGPD; glycerol-3-phosphate dehydrogenase 2 (mitochondrial); mtGPD

products gene name :

GPD2

other gene names :

GPD2; GPD2; GDH2; GPDM; mGPDH; GPD-M; GPDH-M

uniprot entry name :

GPDM_HUMAN

host :

E Coli

sequence length :

727

purity :

Greater than 95.0% as determined by SDS-PAGE.

form :

GPD2 protein solution (0.095mg/ml) is supplied in 20mM Tris-HCl pH 8, 1mM EDTA and 50% glycerol.

concentration :

0.095mg/ml

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Please avoid freeze thaw cycles.

products categories :

ENZYMES; Enzymes; Dehydrogenase

products description :

Description: GPD2 Human Recombinant produced in E Coli is single, a non-glycosylated, Polypeptide chain containing 558 amino acids fragment (43-600) corresponding to the GlpA domain fragment of the mature protein, having a total molecular mass of 64kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. The GPD2 is purified by proprietary chromatographic techniques. Introduction: GPD2 (Mitochondrial glycerol-3-phosphate dehydrogenase) is a Ca2+-sensitive, FAD-binding protein, which is located on the outer surface of the inner mitochondrial membrane. Two isoforms have been identified for mGPD: Isoform 1 is comprised of 727 a.a. residues, while isoform 2 lacks 126 a.a. residues of the N-terminus. GPD2 catalyses the oxidation of glycerol-3-phosphate to DHAP (dihydroxyacetone phosphate) with associated reduction of the enzyme-bound FAD. GPD2 is a testis-specific promoter of mitochondrial GPDH. GPD2 along with a cytosolic NAD-linked GPD forms the glycerol phosphate shuttle that uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, which results in the reoxidation of NADH produced during glycolysis.GPD2 deficiency contributes to the impairment of glucose-stimulated insulin discharge in a number of animal models of non-insulin dependent diabetes mellitus. GPD2 up-regulation as a result of a highly glycolytic environment contributes to the general increase in ROS generation and may lead to the progression of prostate cancer.

ncbi gi num :

285002233

ncbi acc num :

NP_000399.3

ncbi gb acc num :

NM_000408.4

uniprot acc num :

P43304

ncbi mol weight :

67,531 Da

ncbi pathways :

Fatty Acid Beta Oxidation Pathway (198865); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Glycerol Degradation Pathway (142310); Glycerol-3-phosphate Shuttle Pathway (545367); Glycerol-3-phosphate Shuttle Pathway (139739)

ncbi summary :

The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010]

uniprot summary :

GPD2: localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010]. Protein type: EC 1.1.5.3; Oxidoreductase; Lipid Metabolism - glycerophospholipid; Mitochondrial; Calcium-binding. Chromosomal Location of Human Ortholog: 2q24.1. Cellular Component: glycerol-3-phosphate dehydrogenase complex; mitochondrial inner membrane. Molecular Function: glycerol-3-phosphate dehydrogenase activity; calcium ion binding. Biological Process: glycerol-3-phosphate metabolic process; camera-type eye development; multicellular organism growth; cellular lipid metabolic process; gluconeogenesis; glycerol catabolic process. Disease: Diabetes Mellitus, Noninsulin-dependent

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

4630

more info or order :

MyBioSource product webpage