protein

Recombinant Human Thymidylate Synthetase

MBS143614

1 mg

2220 USD

Recombinant Protein

Recombinant Human Thymidylate Synthetase

Thymidylate Synthetase

TYMS Human; Thymidylate Synthetase Human Recombinant; TMS; EC 2.1.1.45; HST422; Thymidylate synthase; TSase; TS; TYMS; MGC88736

thymidylate synthase; Thymidylate synthase; thymidylate synthase; TSase; thymidylate synthetase

TYMS

TYMS; TYMS; TS; TMS; HST422; TS; TS; TSase

TYSY_HUMAN

E Coli

313

MGSSHHHHHH SSGLVPRGSH MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG YNPHPTIKME MAV.

Greater than 95.0% as determined by SDS-PAGE.

The Thymidylate synthase solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol. Sterile Filtered colorless solution.

Thymidylate synthase although stable 4 degree C for 4 weeks, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

ENZYMES; Enzymes; Synthetase

Description: Thymidylate synthase Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 333 amino acids (1-313 a.a.) and having a molecular mass of 37.8 kDa. The Thymidylate synthase fused to a 20 amino acid His-Tag at N-Terminus and purified by proprietary chromatographic techniques. Introduction: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate as a cofactor which maintains the dTMP (thymidine-5-prime monophosphate) pool vital for DNA replication and repair. Thymidylate synthase plays an important role as a cancer chemotherapeutic agent. Thymidylate synthase is the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and several folate analogs.

4507751

NP_001062.1

NM_001071.2

P04818

26,140 Da

Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!E2F Mediated Regulation Of DNA Replication Pathway 105778!!E2F Transcription Factor Network Pathway 137934!!Fluoropyrimidine Activity Pathway 198778!!G1/S Transition Pathway 105769!!G1/S-Specific Transcription Pathway 105776!!Integrated Pancreatic Cancer Pathway 711360!!Metabolic Pathways 132956!!Metabolism Pathway 477135

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008]

1 mg

2220 USD