protein

Recombinant Human Integrin Linked Kinase

MBS143609

0.002 mg

140 USD

Recombinant Protein

Recombinant Human Integrin Linked Kinase

Integrin Linked Kinase

ILK1 Human; Integrin Linked Kinase Human Recombinant; Integrin-linked protein kinase; ILK-1; ILK-2; 59 kDa serine/threonine-protein kinase; p59ILK; ILK; ILK1; ILK2; DKFZp686F1765; P59

integrin-linked protein kinase isoform 1; Integrin-linked protein kinase; integrin-linked protein kinase; 59 kDa serine/threonine-protein kinase; epididymis secretory protein Li 28; integrin-linked kinase-2; integrin-linked kinase; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK

ILK1

ILK; ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28; ILK1; ILK2

ILK_HUMAN

E Coli

452

Greater than 95.0% as determined by SDS-PAGE.

ILK1 protein is supplied in 25mM Sodium Acetate (pH 4.8) and 50% glycerol. Sterile Filtered clear solution.

Quantity Shipped: 10 ug/106 ul

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Please avoid freeze thaw cycles.

PROTEIN KINASES; Enzymes; Protein Kinases

Description: ILK1 Human Recombinant produced in E Coli is single, a non-glycosylated, Polypeptide chain containing 452 amino acids fragment (1-452) having a molecular mass of 55.92kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. The ILK1 is purified by proprietary chromatographic techniques. Introduction: ILK1 (Integrin-linked kinase) is a serine/threonine protein kinase, containing 4 ankyrin-like repeats. ILK1 regulates a number of biological properties which include: anchorage-independent cell cycle progression, tumor cell invasion and apoptosis. ILK1 can also be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Furthermore, ILK1 phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.ILK1 interacts with the cytoplasmic domains of integrin ?1 and ?3 subunits in addition to several adaptors and signaling proteins, it also acts as a proximal receptor kinase regulating integrin-mediated signal transduction. ILK1 is a focal adhesion protein part of the complex ILK-PINCH. This complex is deemed to be one of the convergence points of integrin- and growth factor-signaling pathway. ILK1 is stimulated rapidly but briefly by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, probably through the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK.ILK1 over-expression has been documented in a wide variety of human malignancies.

62420873

NP_001014794.1

NM_001014794.2

Q13418

36,467 Da

Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-extracellular Matrix Interactions Pathway (160967); Endometrial Cancer Pathway (83109); Endometrial Cancer Pathway (521); Eukaryotic Transcription Initiation Pathway (198917); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067)

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle. Protein type: EC 2.7.11.1; Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; ILK subfamily. Chromosomal Location of Human Ortholog: 11p15.4. Cellular Component: protein complex; focal adhesion; costamere; terminal button; intercellular junction; cytosol; nucleoplasm; sarcomere; cell soma; membrane; lamellipodium; cytoplasm; plasma membrane; stress fiber; dendritic shaft; cell junction. Molecular Function: integrin binding; protein serine/threonine kinase activity; signal transducer activity; protein binding; protein kinase binding; SH3 domain binding; ATP binding. Biological Process: negative regulation of smooth muscle cell proliferation; protein heterooligomerization; positive regulation of transcription, DNA-dependent; positive regulation of axon extension; cell-matrix adhesion; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of MAP kinase activity; myelin formation; positive regulation of cell proliferation; protein kinase B signaling cascade; fibril organization and biogenesis; negative regulation of neuron apoptosis; cell cycle arrest; positive regulation of cell-matrix adhesion; positive regulation of BMP signaling pathway; integrin-mediated signaling pathway; cell aging; positive regulation of dendrite morphogenesis; positive regulation of myoblast differentiation; myelination in the peripheral nervous system; establishment and/or maintenance of epithelial cell polarity; positive regulation of osteoblast differentiation; cell proliferation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; ureteric bud branching; regulation of actin cytoskeleton organization and biogenesis; negative regulation of protein kinase activity; nerve development; neurite morphogenesis; positive regulation of phosphorylation; positive regulation of cell migration

0.002 mg

140 USD

0.01 mg

205

1 mg

4630