protein

Recombinant Human Glutathione S-Transferase pi 1

MBS143603

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Glutathione S-Transferase pi 1

Glutathione S-Transferase pi 1

GSTP1 Human; Glutathione S-Transferase pi 1 Human Recombinant; Glutathione S-transferase P; GST class-pi; GSTP1-1; GSTP1; FAEES3; GST3; PI; DFN7

glutathione S-transferase P; Glutathione S-transferase P; glutathione S-transferase P; GST class-pi; GSTP1-1; deafness, X-linked 7; epididymis secretory protein Li 22; fatty acid ethyl ester synthase III; glutathione S-transferase pi 1; GST class-pi; GSTP1-1

GSTP1

GSTP1; GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22; FAEES3; GST3

GSTP1_HUMAN

E Coli

210

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP YTVVYFPVRG RCAALRMLLA DQGQSWKEEV VTVETWQEGS LKASCLYGQL PKFQDGDLTL YQSNTILRHL GRTLGLYGKD QQEAALVDMV NDGVEDLRCK YISLIYTNYE AGKDDYVKAL PGQLKPFETL LSQNQGGKTF IVGDQISFAD YNLLDLLLIH EVLAPGCLDA FPLLSAYVGR LSARPKLKAF LASPEYVNLP INGNGKQ.

Greater than 95.0% as determined by SDS-PAGE.

The GSTP1 solution contains 20mM Tris-HCl buffer (pH7.0), 30% glycerol, 1mM EDTA and 0.1mM PMSF. Sterile Filtered colorless solution.

20ug/40ul

Biological Activity: Specific activity is < 6.5 units/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

ENZYMES; Enzymes; Transferase

Description: GSTP1 Human Recombinant fused with 37 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 247 amino acids (1-210 a.a.) and having a molecular mass of 27.6kDa.The GSTP1 is purified by proprietary chromatographic techniques. Introduction: GSTP1 is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are believed to function in xenobiotic metabolism and have a part in susceptibility to cancer, and other diseases. GSTP1 is a glutathione S-transferase belonging to the pi class. GST family enzymes play a significant role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based upon the biochemical, immunologic and structural properties of the soluble GSTs they are grouped into 4 main classes: alpha, mu, pi, and theta. The GSTP1 enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form a Sulfur-substituted glutathione. The reactions employing glutathione contribute the transformation of a broad range of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress.The GSTP1 inactivation through CpG hypermethylation is frequent in pituitary adenomas and may be a factor in aggressive pituitary tumor behavior. GSTP1 is may be a transcriptional target of the p53 tumor suppressor gene. Single-nucleotide polymorphism in GSTP1 is linked to modified protein binding, which influence GSTP1's contribution to carcinogen and drug metabolism, and possibly disease pathogenesis and/or drug response. GST-pi might have central roles in proliferation of androgen-independent human prostate cancer cells.

4504183

NP_000843.1

NM_000852.3

P09211

23,356 Da

4-hydroxy-2-nonenal Detoxification Pathway (703096); Arachidonate Epoxygenase / Epoxide Hydrolase Pathway (198838); Biological Oxidations Pathway (105698); Cellular Responses To Stress Pathway (645258); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1127658); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1127659); Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway (1127660)

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]

GSTP1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. Homodimer. Interacts with CDK5. Belongs to the GST superfamily. Pi family. Protein type: Transferase; EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: extracellular space; mitochondrion; cytoplasm; plasma membrane; intracellular; nucleus; cytosol; vesicle. Molecular Function: JUN kinase binding; protein binding; glutathione transferase activity; kinase regulator activity. Biological Process: negative regulation of MAP kinase activity; negative regulation of JNK activity; central nervous system development; negative regulation of MAPKKK cascade; negative regulation of acute inflammatory response; negative regulation of interleukin-1 beta production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of tumor necrosis factor production; negative regulation of stress-activated MAPK cascade; regulation of stress-activated MAPK cascade; negative regulation of biosynthetic process; response to reactive oxygen species; glutathione metabolic process; negative regulation of nitric-oxide synthase biosynthetic process; negative regulation of fibroblast proliferation; xenobiotic metabolic process; positive regulation of superoxide release; negative regulation of protein kinase activity; negative regulation of apoptosis

0.005 mg

140 USD

0.02 mg

205

1 mg

3490