product summary
request information :
company name :
MyBioSource
product type :
protein
product name :
Recombinant Ovine Leptin, MTS tag
catalog :
MBS143593
quantity :
0.02 mg
price :
140 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS143593
products type :
Recombinant Protein
products full name :
Recombinant Ovine Leptin, MTS tag
products short name :
Leptin
products name syn :
Leptin Ovine, MTS; Leptin Ovine Recombinant, MTS tag; OB Protein; Obesity Protein; OBS; Obesity factor; oLeptin, MTS
other names :
Leptin; Leptin; leptin; leptin (obesity homolog, mouse); Obesity factor
other gene names :
LEP; LEP; ob; OB
uniprot entry name :
LEP_SHEEP
host :
E Coli
sequence length :
146
sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg.
purity :
Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
form :
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Leptin Ovine MTS tagged although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Leptin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Leptin in sterile 0.02% NaHCO3 not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC,using calibrated solution of Leptin Ovine as a Reference Standard. Biological Activity: Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
products categories :
CYTOKINES AND GROWTH FACTORS; Growth Factors; Leptin
products description :
Description: Leptin Ovine MTS tagged Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17.5 kDa.The Leptin is purified by proprietary chromatographic techniques. The membrane translocating sequence Tag is composed of 10 amino acids Val-Leu-Leu-Pro-Val-Leu-Leu-Ala-Ala-Pro located at the N-terminus. Introduction: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.
ncbi gi num :
3041703
ncbi acc num :
Q28603.2
uniprot acc num :
Q28603
ncbi mol weight :
16,054 Da
ncbi pathways :
AMPK Signaling Pathway 1030771!!AMPK Signaling Pathway 992181!!Adipocytokine Signaling Pathway 1030837!!Adipocytokine Signaling Pathway 505!!Cytokine-cytokine Receptor Interaction Pathway 1030752!!Cytokine-cytokine Receptor Interaction Pathway 460!!Jak-STAT Signaling Pathway 1030801!!Jak-STAT Signaling Pathway 488!!Neuroactive Ligand-receptor Interaction Pathway 1030753!!Neuroactive Ligand-receptor Interaction Pathway 462
size :
0.02 mg
price :
140 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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