protein

Recombinant Human Pituitary Tumor-Transforming Protein 1

MBS143583

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Pituitary Tumor-Transforming Protein 1

Pituitary Tumor-Transforming Protein 1

PTTG1 Human; Pituitary Tumor-Transforming Protein 1 Human Recombinant; Securin; Pituitary tumor-transforming gene 1 protein; hPTTG; Tumor-transforming protein 1; Esp1-associated protein; PTTG1; EAP1; PTTG; TUTR1; MGC126883; MGC138276

securin; Securin; securin; ESP1-associated protein 1; pituitary tumor-transforming gene 1 protein; tumor-transforming protein 1; pituitary tumor-transforming 1; Esp1-associated protein; Pituitary tumor-transforming gene 1 protein; Tumor-transforming protein 1; hPTTG

PTTG1

PTTG1; PTTG1; EAP1; PTTG; HPTTG; TUTR1; EAP1; PTTG; TUTR1; Tumor-transforming protein 1; hPTTG

PTTG1_HUMAN

E Coli

202

MGSSHHHHHH SSGLVPRGSH MATLIYVDKE NGEPGTRVVA KDGLKLGSGP SIKALDGRSQ VSTPRFGKTF DAPPALPKAT RKALGTVNRA TEKSVKTKGP LKQKQPSFSA KKMTEKTVKA KSSVPASDDA YPEIEKFFPF NPLDFESFDL PEEHQIAHLP LSGVPLMILD EERELEKLFQ LGPPSPVKMP SPPWESNLLQ SPSSILSTLD VELPPVCCDI DI.

Greater than 80.0% as determined by SDS-PAGE.

The PTTG1 solution contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl and transforming gene 10% glycerol. Sterile Filtered colorless solution.

PTTG1 although stable 4 degree C for 4 weeks, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins

Description: PTTG1 Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 222 amino acids (1- 202 a.a.) and having a molecular mass of 24.1kDa.The PTTG1 is purified by proprietary chromatographic techniques. Introduction: PTTG1 (Securin) is mainly involved in the regulation of sister chromatid separation during cell division. Securin is a regulatory protein, which has a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Additional two roles have been identified for PTTG1; the first one is to facilitate the transport of separase (cysteine protease) to the nucleus and the second role is to hinder the catalytic activity of separase. PTTG1 is ubiquitinated by the APC (Anaphase Promoting Complex), and subsequently degraded by the Proteasome, releasing separase. During the mitosis, PTTG1 blocks Separase/ESPL1 function, thus preventing the proteolysis of the cohesin complex and the ensuing segregation of the chromosomes. However, PTTG1 function is not restricted to a blocking activity only, since it is required to activate ESPL1. PTTG1 is ubiquitinated at the beginning of anaphase, leading to its destruction and to the liberation of ESPL1. PTTG1 contains two PXXP motifs, which are necessary for its transforming and tumorigenic activities, in addition to its stimulation of basic fibroblast growth factor expression. It also contains a D box (destruction box) which is essential for its degradation by the APC. The acidic C-terminal region of the Securin can function as a transactivation domain. Even though, PTTG1 is primarily a cytosolic protein, it partially localizes in the nucleus.PTTG1 is highly expressed in various tumors; it also has transforming activity in vitro and tumorigenic activity in vivo. Furthermore, PTTG1 negatively regulates the transcriptional activity and related apoptosis activity of TP53.

538260592

NP_001269311.1

NM_001282382.1

O95997

22,024 Da

APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); APC/C:Cdc20 Mediated Degradation Of Securin Pathway (105833); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105831); APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway (105835); Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105829); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463)

The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]

Securin: Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation. Belongs to the securin family. Protein type: Cell cycle regulation; Oncoprotein. Chromosomal Location of Human Ortholog: 5q35.1. Cellular Component: cytoplasm; nucleus; cytosol. Molecular Function: protein binding; cysteine protease inhibitor activity; SH3 domain binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; mitosis; regulation of transcription, DNA-dependent; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; spermatogenesis; mitotic cell cycle; DNA repair; chromosome organization and biogenesis; chromosome segregation

0.005 mg

140 USD

0.02 mg

205

1 mg

3490