protein

Recombinant Human Non-Metastatic Cells 1

MBS143549

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Non-Metastatic Cells 1

Non-Metastatic Cells 1

NME1 Human; Non-Metastatic Cells 1 Human Recombinant; Nucleoside diphosphate kinase A; NDP kinase A; NDK A; Tumor metastatic process-associated protein; Metastasis inhibition factor nm23; nm23-H1; Granzyme A-activated DNase; GAAD; NME1; NDPKA; NM23; NB; AWD; NBS; NDPK-A

nucleoside diphosphate kinase A isoform b; Nucleoside diphosphate kinase A; nucleoside diphosphate kinase A; NDP kinase A; granzyme A-activated DNase; metastasis inhibition factor nm23; non-metastatic cells 1, protein (NM23A) expressed in; tumor metastatic process-associated protein; NME/NM23 nucleoside diphosphate kinase 1; Granzyme A-activated DNase; GAAD; Metastasis inhibition factor nm23; NM23-H1; Tumor metastatic process-associated protein

NME1

NME1; NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1; NDPKA; NM23; NDK A; NDP kinase A; GAAD

NDKA_HUMAN

E Coli

152

MANCERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF MQASEDLLKE HYVDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFHPEELV DYTSCAQNWI YE.

Greater than 95.0% as determined by SDS-PAGE.

The NME1 solution contains 20mM Tris-HCl buffer (pH7.5), 1mM DTT and 10% glycerol. Sterile Filtered colorless solution.

20ug/20ul

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Non-Metastatic Cells

Description: NME1 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 152 amino acids (1-152 a.a.) and having a molecular mass of 17.1kDa.The NME1 is purified by proprietary chromatographic techniques. Introduction: NDK (Nucleoside diphosphate kinase) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by NME2) isoforms. NME1 is involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. It also has tumor metastasis-suppressive capacity. NME1 has a key role in the synthesis of nucleoside triphosphates other than ATP. NME1 is essential for neural development including neural patterning and cell future determination. The NME1 gene is expressed in various tumor types where its levels have been alternatively linked to reduced or increased metastatic potential. Decrease in NME1 expression is notably connected to aggressive behavior in melanoma, breast, colon, and gastric carcinomas. In contrast, elevated levels of NME1 gene expression are noted in the advanced stage of thyroid carcinomas.Somatic mutations of the NME1 gene are found in neuroblastoma. Increased NME1 in neuroblastoma is linked to features of the disease that are associated with aggressive tumors.

4557797

NP_000260.1

NM_000269.2

P15531

30,137 Da

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (618580); Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (468242); Arf6 Downstream Pathway (138038); Arf6 Trafficking Events Pathway (137954); CMP Phosphorylation Pathway (782393); CMP Phosphorylation Pathway (908070); E-cadherin Signaling In The Nascent Adherens Junction Pathway (138005); Guanine Ribonucleotide Biosynthesis IMP = GDP,GTP Pathway (618581); Guanine Ribonucleotide Biosynthesis IMP = GDP,GTP Pathway (468243); Integrated Pancreatic Cancer Pathway (711360)

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]

NME1: an enzyme with multiple activities including histidine protein kinase, nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase and 3 -5 exonuclease activities. Plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Involved in cell proliferation, differentiation and development, G protein-coupled receptor endocytosis, cytotoxic T cell and NK cell-mediated cell death, and gene expression. Required for neural development including neural patterning and cell fate determination. During granzyme A (GZMA)-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3 end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Three alternatively spliced human proteins have been reported. Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Kinase, nucleoside diphosphate; Tumor suppressor; EC 2.7.4.6; Protein kinase, histidine; Kinase, other; Other group; NDK family. Chromosomal Location of Human Ortholog: 17q21.3. Cellular Component: mitochondrion; membrane; cytoplasm; nucleus; cytosol. Molecular Function: identical protein binding; protein binding; GTP binding; magnesium ion binding; deoxyribonuclease activity; nucleoside diphosphate kinase activity; ATP binding; ribosomal small subunit binding. Biological Process: GTP biosynthetic process; nervous system development; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; UTP biosynthetic process; nucleoside diphosphate phosphorylation; endocytosis; DNA catabolic process; regulation of apoptosis; negative regulation of cell proliferation; CTP biosynthetic process; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; cell differentiation; positive regulation of epithelial cell proliferation; purine nucleotide metabolic process; positive regulation of DNA binding. Disease: Neuroblastoma, Susceptibility To

0.005 mg

140 USD

0.02 mg

205

1 mg

2665