catalog number :
MBS143539
products type :
Recombinant Protein
products full name :
Recombinant Rat Carboxypeptidase-B
products short name :
Carboxypeptidase-B
products name syn :
Carboxypeptidase B Rat; Carboxypeptidase-B Rat Recombinant; Carboxypeptidase B; Cpb1; Cpb; rCarboxypeptidase-B
other names :
carboxypeptidase B; Carboxypeptidase B; carboxypeptidase B; pancreatic carboxypeptidase B1; carboxypeptidase B1 (tissue)
other gene names :
Cpb1; Cpb1; Cpb; Cpb
uniprot entry name :
CBPB1_RAT
purity :
Greater than 90% as determined by SDS-PAGE.
form :
The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5. Sterile Filtered lyophilized powder.
storage stability :
Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
other info1 :
Unit Defenition: One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25 degree C, pH-7.65.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: 170 units/mg protein.
products categories :
ENZYMES; Enzymes; Peptidase
products description :
Description: Recombinant Rat Carboxypeptidase-B is expressed in E Coli having a Mw of 35kDa is purified by standard chromatography techniques. Recombinant Rat Carboxypeptidase-B is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Carboxypeptidase-B is free from protease inhibitors such as PMSF and EDTA. Introduction: Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 34.5 kda, optimun pH-7.9, and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA, 1,10-phenanthroline.
ncbi acc num :
NP_036665.1
ncbi gb acc num :
NM_012533.1
ncbi mol weight :
47,515 Da
ncbi pathways :
Pancreatic Secretion Pathway 169303!!Pancreatic Secretion Pathway 169295!!Protein Digestion And Absorption Pathway 172816!!Protein Digestion And Absorption Pathway 171868
ncbi summary :
putative carboxypeptidase B protein [RGD, Feb 2006]