protein

Recombinant Human Aldo-Keto Reductase Family 1 Member C3

MBS143533

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Aldo-Keto Reductase Family 1 Member C3

Aldo-Keto Reductase Family 1 Member C3

AKR1C3 Human; Aldo-Keto Reductase Family 1 Member C3 Human Recombinant; DD3; DDX; HAKRB; HAKRe; HA1753; HSD17B5; hluPGFS; KIAA0119; AKR1C3; Aldo-keto reductase family 1 member C3; 3-alpha-HSD type 2; 17-beta-HSD 5; PGFS; DD-3

aldo-keto reductase family 1 member C3 isoform 2; Aldo-keto reductase family 1 member C3; aldo-keto reductase family 1 member C3; 3-alpha hydroxysteroid dehydrogenase, type II; 3-alpha-HSD type II, brain; aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II); chlordecone reductase homolog HAKRb; dihydrodiol dehydrogenase 3; dihydrodiol dehydrogenase X; indanol dehydrogenase; prostaglandin F synthase; testosterone 17-beta-dehydrogenase 5; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type IIb 3-alpha hydroxysteroid dehydrogenase; aldo-keto reductase family 1, member C3; 17-beta-hydroxysteroid dehydrogenase type 5; 17-beta-HSD 5; 3-alpha-HSD type II, brain; 3-alpha-hydroxysteroid dehydrogenase type 2 (EC:1.1.1.357); 3-alpha-HSD type 2; Chlordecone reductase homolog HAKRb; Dihydrodiol dehydrogenase 3; DD-3; DD3; Dihydrodiol dehydrogenase type I; HA1753; Indanol dehydrogenase (EC:1.1.1.112); Prostaglandin F synthase (EC:1.1.1.188); PGFS; Testosterone 17-beta-dehydrogenase 5 (EC:1.1.1.239, EC:1.1.1.64); Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (EC:1.3.1.20)

AKR1C3

AKR1C3; AKR1C3; DD3; DDX; PGFS; HAKRB; HAKRe; HA1753; HSD17B5; hluPGFS; DDH1; HSD17B5; KIAA0119; PGFS; 17-beta-HSD 5; 3-alpha-HSD type 2; DD-3; DD3; PGFS

AK1C3_HUMAN

E Coli

323

MGSSHHHHHH SSGLVPRGSH MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKPGLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY.

Greater than 95.0% as determined by SDS-PAGE.

The AKR1C3 solution (1mg/ml) contains 20mM Tris-HCl pH-8 and 10% glycerol. Sterile Filtered clear colorless solution.

1mg/ml

AKR1C3 Recombinant Human although stable at 4 degree C for 30 days, should be stored desiccated below -20 degree C for periods greater than 30 days. Please avoid freeze-thaw cycles.

Biological Activity: Specific activity: approximately < 0.1 units/mg.Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25C. Specific activity was expressed as units/mg protein.

ENZYMES; Enzymes; Reductase

Description: AKR1C3 Human Recombinant fused to 20 amino acid His Tag at N-terminal produced in E Coli is a single, non-glycosylated, polypeptide chain containing 343 amino acids (1-323 a.a.) and having a molecular mass of 39 kDa. The AKR1C3 is purified by proprietary chromatographic techniques. Introduction: AKR1C3 is part of the aldo/keto reductase superfamily, which has at least 40 identified proteins. AKR1C3 catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. AKR1C3 displays overlapping but distinct substrate specificity. AKR1C3 catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. AKR1C3 is involved in the pathogenesis of allergic diseases such as asthma. AKR1C3 controls cell growth and/or differentiation. AKR1C3, having ability to convert androstenedione to testosterone, itakes part in adrenal testosterone production. AKR1C3 expression is affected by metabolic disease, and its levels are considerably reduced in response to diet-induced weight loss and correlate with leptin levels.

359806990

NP_001240837.1

NM_001253908.1

P42330

23,364 Da

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Benzo(a)pyrene Metabolism Pathway (198911); Bile Acid And Bile Salt Metabolism Pathway (106144); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

AKR1C3: Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta- PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone. Belongs to the aldo/keto reductase family. Protein type: EC 1.1.1.149; Xenobiotic Metabolism - metabolism by cytochrome P450; Oxidoreductase; Lipid Metabolism - arachidonic acid. Chromosomal Location of Human Ortholog: 10p15-p14. Cellular Component: cytoplasm; intracellular; cytosol; nucleus. Molecular Function: 15-hydroxyprostaglandin-D dehydrogenase (NADP+) activity; delta4-3-oxosteroid 5beta-reductase activity; aldo-keto reductase activity; prostaglandin-F synthase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; phenanthrene 9,10-monooxygenase activity; retinol dehydrogenase activity; 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; testosterone 17-beta-dehydrogenase (NADP+) activity; ketosteroid monooxygenase activity; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; ketoreductase activity; indanol dehydrogenase activity; aldehyde reductase activity; geranylgeranyl reductase activity; retinal dehydrogenase activity. Biological Process: steroid metabolic process; phototransduction, visible light; retinal metabolic process; cyclooxygenase pathway; male gonad development; progesterone metabolic process; cellular response to starvation; prostaglandin metabolic process; keratinocyte differentiation; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; protein import into nucleus, translocation; positive regulation of cell proliferation; arachidonic acid metabolic process; farnesol catabolic process; multicellular organismal macromolecule metabolic process; retinoid metabolic process; regulation of retinoic acid receptor signaling pathway; response to nutrient

0.005 mg

140 USD

0.02 mg

205

1 mg

3490