protein

Recombinant Rat Interleukin-17 A/F Heterodimer

MBS143518

0.01 mg

205 USD

Recombinant Protein

Recombinant Rat Interleukin-17 A/F Heterodimer

Interleukin-17 A/F

IL 17A/F Rat; Interleukin-17A/F Heterodimer Rat Recombinant; IL17A/F; IL17 A/F; IL-17A/F; IL-17 A/F; IL17AF; IL-17 AF; Interleukin-17 A/F; Interleukin-17 AF

interleukin 17A, partial; Interleukin-17A; interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8

rIL 17 A/F

IL17A; IL17A; IL17; CTLA8; IL-17; IL-17A; CTLA8; IL17; IL-17; IL-17A; CTLA-8

IL17_HUMAN

E Coli

46

Greater than 98.0% as determined by HPLC and SDS-PAGE.

Lyophilized from a concentrated (1mg/ml) solution containing no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Rat IL-17A/F although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Rat IL-17A/F should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Rat IL-17A/F in sterile water not more than 1mg/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 as determined by its ability to induce of IL-6 production from NIH-3T3 cells, is 15.3-23 ng/ml.

CYTOKINES AND GROWTH FACTORS; Cytokines; Interleukin

Description: IL-17A/F Rat Recombinant produced in E Coli is a heterodimeric, non-glycosylated polypeptide chain containing 1 monomeric subunit of each IL-17A & IL-17F. The dimer contains 269 amino acids and having a total molecular mass of 30.7 kDa. The IL-17A/F Rat is purified by proprietary chromatographic techniques. Introduction: Rat IL-17A/F is a glycoprotein which is secreted as a disulfide-linked heterodimer. IL-17A/F consists of two proteins of the IL-17 family, IL-17A and IL17F. Proteins of the 6 homodimeric IL17 family show a cysteine knot motif that contains two disulfide-bonds. Rat IL17A is produced as a 155 a.a precursor that includes a 23 amino acids signal sequence and a 132 amino acid chain that includes an N-linked glycosylation site. Rat IL17F is produced as a 153 amino acid precursor with a 20 amino acid signal sequence and a 133 amino acid region. Similar to IL17A, IL17F also has an N-linked glycosylation site. Both proteins (IL17A & IL17F) share 50% amino acid sequence identity. Human IL17A & IL17F show approximately 60% homology in their amino acid sequence to mouse IL-17A and IL-17F. Interleukin-17A/F and IL17A, IL17F homodimers are manufactured by activted CD4+ T cells, called Th17. IL-23 causes Th17 lymphocytes to manufacture IL-17A/F. IL17RA and IL17RC form a heterodimer for the binding of IL17A and IL17F. IL-17A/F binds IL-17RA. Interleukin-17A/F induces chemokine production and airway neutrophilia with intermediate potency between IL17A (most potent) and IL17F (least potent).

299832923

ADJ56412.1

17,504 Da

Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!IL17 Signaling Pathway 920968!!IL23-mediated Signaling Events Pathway 138000!!IL27-mediated Signaling Events Pathway 137929!!Inflammatory Bowel Disease (IBD) Pathway 842771!!Inflammatory Bowel Disease (IBD) Pathway 842797!!Rheumatoid Arthritis Pathway 200309!!Rheumatoid Arthritis Pathway 200269!!TCR Signaling Pathway 198862

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

0.01 mg

205 USD