protein

Recombinant Human Lactate Dehydrogenase A

MBS143494

1 mg

2220 USD

Recombinant Protein

Recombinant Human Lactate Dehydrogenase A

Lactate Dehydrogenase A

LDHA Human; Lactate Dehydrogenase A Human Recombinant; LDH-A; GSD11; LDH1; LDHM; PIG19; EC 1.1.1.27; lactate dehydrogenase M; LDH-M; LDH-1; L-lactate dehydrogenase A chain; LDH muscle subunit; Renal carcinoma antigen NY-REN-59; Cell proliferation-inducing gene 19 protein; LDHA

L-lactate dehydrogenase A chain isoform 2; L-lactate dehydrogenase A chain; L-lactate dehydrogenase A chain; LDH muscle subunit; LDH-A; LDH-M; cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; lactate dehydrogenase M; renal carcinoma antigen NY-REN-59; lactate dehydrogenase A; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59

LDHA

LDHA; LDHA; LDH1; LDHM; GSD11; PIG19; HEL-S-133P; LDH-A; LDH-M

LDHA_HUMAN

E Coli

274

MGSSHHHHHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF.

Greater than 95.0% as determined by SDS-PAGE.

The LDHA protein solution contains 20mM Tris-HCl pH-8, 100mM NaCl and 20% glycerol. Sterile Filtered colorless solution.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Biological Activity: Specific activity is > 20 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37 degree C.

ENZYMES; Enzymes; Dehydrogenase

Description: LDHA Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 352 amino acids (1-332 a.a.) and having a molecular mass of 38.8 kDa. The LDHA is fused to a 20 amino acid his tag at N-terminus and purified by conventional chromatography. Introduction: LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential.

207028494

NP_001128711.1

NM_001135239.1

P00338

26,712 Da

Central Carbon Metabolism In Cancer Pathway 1059538!!Central Carbon Metabolism In Cancer Pathway 1084231!!Cysteine And Methionine Metabolism Pathway 104488!!Cysteine And Methionine Metabolism Pathway 103421!!Glycolysis / Gluconeogenesis Pathway 82926!!Glycolysis / Gluconeogenesis Pathway 287!!Glycolysis And Gluconeogenesis Pathway 198814!!HIF-1 Signaling Pathway 695200!!HIF-1-alpha Transcription Factor Network Pathway 138045!!Metabolic Pathways 132956

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]

1 mg

2220 USD