protein

Recombinant Human Macrophage Inflammatory protein-3 (CCL23)

MBS143488

0.02 mg

205 USD

Recombinant Protein

Recombinant Human Macrophage Inflammatory protein-3 (CCL23)

Macrophage Inflammatory protein-3

MIP 3 Human; Macrophage Inflammatory Protein-3 Human Recombinant (CCL23); C-C motif chemokine 23; Small-inducible cytokine A23; Macrophage inflammatory protein 3; Myeloid progenitor inhibitory factor 1; CK-beta-8; MIP-3; MPIF-1; CKB-8; CCL23; MIP3; MPIF1; SCYA23; CKb8; Ckb-8-1

C-C motif chemokine 23 isoform CKbeta8-1; C-C motif chemokine 23; C-C motif chemokine 23; C6 beta-chemokine; macrophage inflammatory protein 3; myeloid progenitor inhibitory factor 1; small inducible cytokine subfamily A (Cys-Cys), member 23; chemokine (C-C motif) ligand 23; CK-beta-8; CKB-8; Macrophage inflammatory protein 3; MIP-3; Myeloid progenitor inhibitory factor 1; MPIF-1; Small-inducible cytokine A23Cleaved into the following 4 chains:CCL23(19-99); CCL23(22-99); CCL23(27-99); CCL23(30-99)

MIP 3

CCL23; CCL23; CKb8; MIP3; Ckb-8; MIP-3; MPIF-1; SCYA23; Ckb-8-1; hmrp-2a; CK-BETA-8; MIP3; MPIF1; SCYA23; CKB-8; MIP-3; MPIF-1

CCL23_HUMAN

E Coli

137

RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESYFETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN.

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Filtered (0.2 um) and lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized MIP-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL23 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized MIP-3 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: Determined by its ability to chemoattract human T cell population using a concentration range of 10-50ng/ml corresponding to a Specific Activity of 20,000-100,000IU/mg.

CHEMOKINES; Chemokines; MIP

Description: MIP-3 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 99 amino acids and having a molecular mass of 11.3kDa. The MIP-3 is purified by proprietary chromatographic techniques. Introduction: CCL23 (MIP-3) is a ligand for the CCR1chemokine receptor. CCL23 is one of several cytokine genes clustered on the q-arm of chromosome 17, in a locus containing several other CC chemokines. MIP-3 chemoattracts monocytes, resting T-lymphocytes and neutrophils, but not activated lymphocytes. Furthermore, it was shown that MIP-3 inhibits colony formation of bone marrow myeloid immature progenitors. MIP-3 is mainly expressed in lung and liver tissue, but can be also found in bone marrow and placenta, as well as in some cell lines of myeloid origin. Alternative splicing of the CCL23 gene produces 2 mRNAs which encode a short (CK?8) and a long (CK?81) isoform of the MIP-3. CK?8 cDNA encodes a 120 amino acid residue precursor protein with a putative 21 a.a. residue signal peptide which is cleaved to generate a 99 a.a. residue mature CK?8 (a.a. 22-120). Further N-terminal processing of the 99 a.a. residue variant can produce a 75 a.a. residue CK?8 (a.a. 46-120) which is considerably more active than the 99 a.a. residue variant.MIP-3 may be involved in the malignant progression of certain human cancer cells which overexpress ErbB2 through the transactivation of ErbB2 tyrosine kinase. MIP-3 may also be involved in angiogenesis via upregulation of matrix metalloproteinase MMP-2 expression.

632794829

NP_005055.3

NM_005064.5

P55773

15,290 Da

Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway 1127664!!Disease Pathway 530764!!Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway 161021!!G Alpha (i) Signalling Events Pathway 119550!!G Alpha (q) Signalling Events Pathway 106043

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils and no activity on activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. In addition, the product of this gene is a potent agonist of the chemokine (C-C motif) receptor 1. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

0.02 mg

205 USD