protein

Recombinant Human Ubiquitin Biotin

MBS143454

0.002 mg

140 USD

Recombinant Protein

Recombinant Human Ubiquitin Biotin

Ubiquitin Biotin

RPS27A Human, Biotin; Ubiquitin Biotinylated Human Recombinant; Ubiquitin; Ribosomal Protein S27a; CEP80; UBA80; UBCEP1; UBCEP80; HUBCEP80; RPS27A; Ubiquitin Biotin

ubiquitin-60S ribosomal protein L40; ubiquitin A-52 residue ribosomal protein fusion product 1

UBA52; L40; CEP52; RPL40; HUBCEP52

E Coli

RPS27A Protein biotinilation is determined by Western Blotting and ELISA analysis using streptavidinHRP conjugated as a detection reagent. Free biotin is eliminated by dialysis against PBS. Protein concentration is determined by 280nm absorbance.

The RPS27A is supplied in 1x PBS and 0.05% PBS. Sterile Filtered colorless liquid formulation.

Lyophilized RPS27A although stable at 15 degree C for 1 week, should be stored desiccated below -18 degree C. For long term storage, to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Ubiquitin

Description: Recombinant Human RPS27A protein biotinylated with NHS-biotin produced in E Coli is a single, non-glycosylated polypeptide chain containing a total of 76 amino acids and having a molecular mass of 8.6 kDa. Introduction: Recombinant Human Ubiquitin having the accession number of P62988 was conjugated to Biotin. RPS27A is a small protein composed of 76 amino acids. RPS27A is found only in eukaryotic organisms among which shows strong sequence conservation. The RPS27A protein is present in all cell types, thus giving rise to its name.RPS27A is found either in free form or conjugated to proteins through a covalent bond between the glycine at the C-terminal end and the side chains of lysine.The connection of multiple copies of RPS27A targets the proteins for degradation by the 26S proteosome. RPS27A ligation is an ATP-dependent multi-step process. RPS27A is activated by the E1 enzyme. The attachment of RPS27A to the target protein is catalyzed by the E2 enzyme acting in concert with E3 which is involved in the recognition of the substrate protein.

51703339

P62988.1

P62988

AMER1 Mutants Destabilize The Destruction Complex Pathway (1127621); APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway (1127616); APC Truncation Mutants Have Impaired AXIN Binding Pathway (1127617); APC-Cdc20 Mediated Degradation Of Nek2A Pathway (1127551); APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway (105832); APC/C:Cdc20 Mediated Degradation Of Securin Pathway (105833); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105831); APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway (105835); APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway (1127549)

Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008]

UBA52: the gene (UBA52) that encodes this protein is one of four that encode for ubiquitin: UBC, UBB, UBA52 and RPS27A. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. The RPS27A gene product is cleaved into the following 2 chains: ubiquitin (amino acids 1-76) and the 60S ribosomal protein L40 (77 ? 128). Ubiquitin is a peptide 76 amino acids in length that can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Hundreds of ubiquitin ligases and hydrolases have been identified, implicating ubiquitin as a major regulatory element in many crucial cellular systems. It can be covalently bound to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. At the protein level, it is not possible to easily determine which of the four genes encoded a given ubiquitin chain. Protein type: Translation; Ribosomal. Chromosomal Location of Human Ortholog: 19p13.1-p12. Cellular Component: nucleoplasm; lysosomal membrane; plasma membrane; ribosome; endosome membrane; cytosol. Molecular Function: protein binding; structural constituent of ribosome. Biological Process: circadian rhythm; SRP-dependent cotranslational protein targeting to membrane; I-kappaB kinase/NF-kappaB cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of apoptosis; activation of MAPK activity; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; endosome transport; T cell receptor signaling pathway; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; transforming growth factor beta receptor signaling pathway; translational initiation; JNK cascade; antigen processing and presentation of exogenous peptide antigen via MHC class I; viral transcription; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; regulation of interferon type I production; glycogen biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; fibroblast growth factor receptor signaling pathway; transcription, DNA-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; Notch receptor processing; glucose metabolic process; virus assembly; toll-like receptor 2 signaling pathway; translational elongation; carbohydrate metabolic process; viral protein processing; mRNA catabolic process, nonsense-mediated decay; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of interferon type I production; negative regulation of apoptosis; G1/S transition of mitotic cell cycle; negative regulation of epidermal growth factor receptor signaling pathway; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; translation; apoptosis; pathogenesis; viral infectious cycle; negative regulation of transcription from RNA polymerase II promoter; translational termination; toll-like receptor 10 signaling pathway; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of interferon type I production; transmembrane transport; epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; protein modification process; DNA repair; MyD88-dependent toll-like receptor signaling pathway; cellular protein metabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; toll-like receptor signaling pathway; innate immune response; gene expression; mitotic cell cycle; negative regulation of transforming growth factor beta receptor signaling pathway

0.002 mg

140 USD

0.01 mg

205

0.1 mg

855