protein

Recombinant Human B-Cell Leukemia/Lymphoma 2 -BH2

MBS143398

0.002 mg

140 USD

Recombinant Protein

Recombinant Human B-Cell Leukemia/Lymphoma 2 -BH2

B-Cell Leukemia/Lymphoma 2 -BH2

Bcl 2 Human (minus BH2 domain); B-Cell Leukemia/Lymphoma 2 Human Recombinant (BH2); Apoptosis regulator Bcl-2; BCL2; B-cell CLL/lymphoma 2; Bcl-2

apoptosis regulator Bcl-2 alpha isoform; Apoptosis regulator Bcl-2; apoptosis regulator Bcl-2; protein phosphatase 1, regulatory subunit 50; B-cell CLL/lymphoma 2

Bcl-2 -BH2

BCL2; BCL2; Bcl-2; PPP1R50

BCL2_HUMAN

E Coli

239

Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Bcl-2 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Western Blot (WB)

Input marker or positive control (Western Blotting).Function study.

Solubility: Suspend Bcl-2 in 100ul of 0.5M Acetic acid, over night at 4 degree C. Dilute 10 fold into selected buffer system.BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; B Cell Lymphoma

Description: Bcl-2 Des BH2 domain (187-202 residues) Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 202 amino acids 1-186 and 203-218.The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques. Introduction: BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

72198189

NP_000624.2

NM_000633.2

P10415

22,337 Da

ATF-2 Transcription Factor Network Pathway (138006); Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Apoptosis Pathway (83060)

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]

Bcl-2: a antiapoptotic member of the Bcl-2 family. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Phosphorylation by JNKs may increase its antiapoptotic functions. Protein type: Membrane protein, integral; Autophagy; Oncoprotein; Apoptosis. Chromosomal Location of Human Ortholog: 18q21.3. Cellular Component: pore complex; endoplasmic reticulum membrane; mitochondrial outer membrane; nuclear membrane; mitochondrion; membrane; endoplasmic reticulum; cytoplasm; nucleus; cytosol; myelin sheath. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protease binding; protein phosphatase 2A binding; protein heterodimerization activity; channel activity; sequence-specific DNA binding; ubiquitin protein ligase binding; BH3 domain binding; channel inhibitor activity; transcription factor binding. Biological Process: response to nicotine; positive regulation of catalytic activity; focal adhesion formation; developmental growth; renal system process; pigment granule organization and biogenesis; protein polyubiquitination; response to toxin; response to glucocorticoid stimulus; T cell differentiation in the thymus; ear development; lymphoid progenitor cell differentiation; positive regulation of multicellular organism growth; female pregnancy; glomerulus development; negative regulation of mitochondrial depolarization; post-embryonic development; cochlear nucleus development; cellular response to glucose starvation; B cell receptor signaling pathway; negative regulation of myeloid cell apoptosis; regulation of mitochondrial membrane potential; negative regulation of ossification; positive regulation of B cell proliferation; regulation of transmembrane transporter activity; T cell homeostasis; negative regulation of neuron apoptosis; cell growth; defense response to virus; spleen development; response to drug; positive regulation of neuron maturation; release of cytochrome c from mitochondria; axon regeneration; regulation of protein homodimerization activity; actin filament organization; cell aging; digestive tract morphogenesis; regulation of calcium ion transport; positive regulation of cell growth; organ growth; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; negative regulation of osteoblast proliferation; gland morphogenesis; regulation of mitochondrial membrane permeability; regulation of nitrogen utilization; metanephros development; oocyte development; negative regulation of apoptosis; B cell proliferation; negative regulation of autophagy; regulation of protein heterodimerization activity; behavioral fear response; melanin metabolic process; regulation of cell-matrix adhesion; apoptosis; negative regulation of retinal cell programmed cell death; regulation of protein stability; positive regulation of smooth muscle cell migration; protein amino acid dephosphorylation; response to radiation; B cell homeostasis; positive regulation of skeletal muscle fiber development; ovarian follicle development; positive regulation of melanocyte differentiation; melanocyte differentiation; response to gamma radiation; response to iron ion; negative regulation of cellular pH reduction; regulation of viral genome replication; negative regulation of cell migration; transmembrane transport; mesenchymal cell development; ossification; CD8-positive, alpha-beta T cell lineage commitment; hair follicle morphogenesis; thymus development; B cell lineage commitment; male gonad development; peptidyl-threonine phosphorylation; positive regulation of peptidyl-serine phosphorylation; humoral immune response; response to UV-B; endoplasmic reticulum calcium ion homeostasis; neuron apoptosis; peptidyl-serine phosphorylation; response to hydrogen peroxide; axonogenesis; homeostasis of number of cells within a tissue; ureteric bud branching; response to cytokine stimulus; innate immune response; negative regulation of cell growth; response to DNA damage stimulus; induction of apoptosis by oxidative stress

0.002 mg

140 USD

0.01 mg

205

1 mg

4630