protein

Recombinant Human p21 Activated Kinase 4

MBS143394

0.005 mg

140 USD

Recombinant Protein

Recombinant Human p21 Activated Kinase 4

p21 Activated Kinase 4

PAK4 Human; p21 Activated Kinase 4 Human Recombinant; PAK-4; p21 (CDKN1A)-Activated Kinase 4; p21-Activated Kinase 4; Serine/threonine-protein kinase PAK 4; KIAA1142; PAK4

serine/threonine-protein kinase PAK 4 isoform 1; Serine/threonine-protein kinase PAK 4; serine/threonine-protein kinase PAK 4; PAK-4; p21(CDKN1A)-activated kinase 4; p21-activated kinase 4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; p21 protein (Cdc42/Rac)-activated kinase 4; p21-activated kinase 4; PAK-4

PAK4

PAK4; PAK4; KIAA1142; PAK-4

PAK4_HUMAN

E Coli

591

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMFG KRKKRVEISA PSNFEHRVHT GFDQHEQKFT GLPRQWQSLI EESARRPKPL VDPACITSIQ PGAPKTIVRG SKGAKDGALT LLLDEFENMS VTRSNSLRRD SPPPPARARQ ENGMPEEPAT TARGGPGKAG SRGRFAGHSE AGGGSGDRRR AGPEKRPKSS REGSGGPQES SRDKRPLSGP DVGTPQPAGL ASGAKLAAGR PFNTYPRADT DHPSRGAQGE PHDVAPNGPS AGGLAIPQSS SSSSRPPTRA RGAPSPGVLG PHASEPQLAP PACTPAAPAV PGPPGPRSPQ REPQRVSHEQ FRAALQLVVD PGDPRSYLDN FIKIGEGSTG IVCIATVRSS GKLVAVKKMD LRKQQRRELL FNEVVIMRDY QHENVVEMYN SYLVGDELWV VMEFLEGGAL TDIVTHTRMN EEQIAAVCLA VLQALSVLHA QGVIHRDIKS DSILLTHDGR VKLSDFGFCA QVSKEVPRRK SLVGTPYWMA PELISRLPYG PEVDIWSLGI MVIEMVDGEP PYFNEPPLKA MKMIRDNLPP RLKNLHKVSP SLKGFLDRLL VRDPAQRATA AELLKHPFLA KAGPPASIVP LMRQNRTR.

Greater than 90% as determined by SDS-PAGE.

The PAK4 protein contains 50mM Tris-HCl pH-8, 2mM DTT and 10% glycerol. Sterile filtered colorless solution.

1mg/ml

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

PROTEIN KINASES; Enzymes; Protein Kinases

Description: PAK4 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 628 amino acids (1-591 a.a.) and having a molecular mass of 68.3 kDa.PAK4 is fused to 37 amino acid His-Tag at N-terminus and is purified by standard chromatography techniques. Introduction: PAK4 is part of the group B family of p21-activated kinases (PAK). It's known as an effector protein for cell division cycle 42(Cdc42) and protein that plays an important role in regulating cytoskeletal organization and cell morphology. PAK4 expression is eminent in many cancer cell lines, and is involved in tumorigenesis. PAK proteins are significant effectors that associate Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins are targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 cooperates specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and is involved in the reorganization of the actin cytoskeleton. PAK4 activates the JNK pathway. PAK4 phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments.

62422554

NP_001014831.1

NM_001014831.2

O96013

54,940 Da

Activation Of Rac Pathway (119530); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); CDC42 Signaling Events Pathway (137994); Developmental Biology Pathway (477129); ErbB Signaling Pathway (198844); ErbB Signaling Pathway (83049); ErbB Signaling Pathway (458); FGF Signaling Pathway (137989)

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

PAK4: a protein kinase of the STE20 family. A mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Interacts specifically with the GTP-bound form of Cdc42 and weakly activates the JNK family of MAP kinases. Overexpressed in several cancers. Required for ras-dependent anchorage-independent growth of tumor cell lines. Four alternatively spliced human isoforms have been reported. Protein type: Protein kinase, STE; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKB subfamily. Chromosomal Location of Human Ortholog: 19q13.2. Cellular Component: Golgi apparatus; focal adhesion. Molecular Function: protein binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity. Biological Process: cell proliferation; cell migration; apoptosis; cytoskeleton organization and biogenesis; mitotic cell cycle; cell growth; signal transduction; cell motility; protein amino acid phosphorylation

0.005 mg

140 USD

0.025 mg

205

1 mg

2780