protein

Recombinant Human Mitogen Activated Kinase Kinase 1

MBS143393

0.05 mg

940 USD

Recombinant Protein

Recombinant Human Mitogen Activated Kinase Kinase 1

Mitogen Activated Kinase Kinase 1

MEK1 Human; Mitogen Activated Kinase Kinase 1 Human Recombinant; MEK1; MKK1; MAPKK1; MAP2K1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; MAPK/ERK kinase 1; PRKMK1

dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1); mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1

MEK1

MAP2K1; MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1; MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1

MP2K1_HUMAN

Sf9 Insect Cells

393

Greater than 90% as determined by SDS-PAGE.

MEK1 is supplied at a concentration of 0.09mg/ml in 40mM Tris, pH-8, 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate and 0.03% Brij-35. Sterile Filtered clear solution.

Store at 4 degree C if entire vial will be used within 1-2 weeks. Store frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

Biological Activity: ~125-175 units/mg. One unit of MEK1 activity transfers 1 nmol of phosphate to ERK1/2 peptide per minute at 30 degree C in a reaction containing 100 uM ATP. Recombinant active MEK1 also phosphorylates ERK1, ERK2, and GSK-3?. Kinase activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application.

PROTEIN KINASES; Enzymes; Protein Kinases

Description: MAP2K1 active Human Recombinant produced in Sf9 cells is a glycosylated, polypeptide chain containing amino acids 2-393 having a molecular mass of 47 kDa. MAP2K1 is fused to a polyhistidine tag and is purified by proprietary chromatographic techniques. Introduction: MAP2K1 is a member of the dual specificity protein kinase family, which plays a role as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, are recognized as extracellular signal-regulated kinases (ERKs), that act as an integration position for multiple biochemical signals. MEK1 is located upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As a key player of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. MAP2K1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a thr-glu-tyr sequence located in map kinases. MEK1 activates erk1 and erk2 map kinases.

5579478

NP_002746.1

NM_002755.3

Q02750

40,764 Da

AGE/RAGE Pathway 698754!!ARMS-mediated Activation Pathway 106466!!Activated TLR4 Signalling Pathway 106400!!Acute Myeloid Leukemia Pathway 83117!!Acute Myeloid Leukemia Pathway 529!!Alcoholism Pathway 585563!!Alcoholism Pathway 587116!!Axon Guidance Pathway 105688!!B Cell Receptor Signaling Pathway 198909!!B Cell Receptor Signaling Pathway 83081

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

0.05 mg

940 USD