Recombinant Human Adenylate Kinase 2 | MyBioSource MBS143387 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Adenylate Kinase 2

catalog :

MBS143387

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS143387

products type :

Recombinant Protein

products full name :

Recombinant Human Adenylate Kinase 2

products short name :

Adenylate Kinase 2

products name syn :

AK2 Human; Adenylate Kinase 2 Human Recombinant; ADK2; AK-2; Adenylate kinase isoenzyme 2 mitochondrial; ATP-AMP transphosphorylase 2; adenylate kinase 2

other names :

adenylate kinase 2, mitochondrial isoform c; Adenylate kinase 2, mitochondrial; adenylate kinase 2, mitochondrial; AK 2; AK2; ATP-AMP transphosphorylase 2; ATP:AMP phosphotransferase; adenylate kinase isoenzyme 2, mitochondrial; adenylate monophosphate kinase; adenylate kinase 2; ATP-AMP transphosphorylase 2; ATP:AMP phosphotransferase; Adenylate monophosphate kinase;

products gene name :

AK2

other gene names :

AK2; AK2; ADK2; AK 2;

uniprot entry name :

KAD2_HUMAN

host :

E Coli

sequence length :

224

sequence :

MGSSHHHHHH SSGLVPRGSH MAPSVPAAEP EYPKGIRAVL LGPPGAGKGTQAPRLAENFC VCHLATGDML RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG FPRTVRQAEM LDDLMEKRKE KLDSVIEFSIPDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKIRLQAY HTQTTPLIEY YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI.

purity :

Greater than 95.0% as determined by SDS-PAGE.

form :

AK2 solution containing 20mM Tris pH-7.5, 5mM DTT and 20% glycerol. Sterile filtered colorless solution.

storage stability :

AK2 Human Recombinant although stable at 4 degree C for 1 week, should be stored below -18 degree C. Please prevent freeze thaw cycles.

other info2 :

Biological Activity: Specific activity: > 1.5 units/ml. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 25C.

products categories :

PROTEIN KINASES; Enzymes; Adenylate Kinase

products description :

Description: AK2 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 259 amino acids and having a molecular mass of 28.6 kDa. AK2 is fused to 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques. Introduction: Adenylate kinases play a role in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. There are 3 types of adenylate kinase isozymes, AK1, AK2, and AK3 in vertebrates. Expression of these isozymes are tissue-specific and developmentally regulated. AK2 is localized in the mitochondrial intermembrane space and is involved in apoptosis. AK2 is mutated in individuals with reticular dysgenesis.

ncbi gi num :

312836806

ncbi acc num :

NP_001186128.1

ncbi gb acc num :

NM_001199199.1

uniprot acc num :

P54819

ncbi mol weight :

21,636 Da

ncbi pathways :

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (618580); Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (468242); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (307); Synthesis And Interconversion Of Nucleotide Di- And Triphosphates Pathway (161057); Adenosine Ribonucleotides De Novo Biosynthesis Pathway (835389); Purine Nucleotides De Novo Biosynthesis Pathway (142277)

ncbi summary :

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.[provided by RefSeq, Nov 2010]

uniprot summary :

AK2: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis. Defects in AK2 are the cause of reticular dysgenesis (RDYS); also known as aleukocytosis. RDYS is the most severe form of inborn severe combined immunodeficiencies (SCID) and is characterized by absence of granulocytes and almost complete deficiency of lymphocytes in peripheral blood, hypoplasia of the thymus and secondary lymphoid organs, and lack of innate and adaptive humoral and cellular immune functions, leading to fatal septicemia within days after birth. In bone marrow of individuals with reticular dysgenesis, myeloid differentiation is blocked at the promyelocytic stage, whereas erythro- and megakaryocytic maturation is generally normal.In addition, affected newborns have bilateral sensorineural deafness. Defects may be due to its absence in leukocytes and inner ear, in which its absence can not be compensated by AK1. Belongs to the adenylate kinase family. AK2 subfamily. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Nucleotide Metabolism - purine; EC 2.7.4.3; Mitochondrial; Kinase, other. Chromosomal Location of Human Ortholog: 1p34. Cellular Component: mitochondrial inner membrane; mitochondrial intermembrane space; cytosol. Molecular Function: adenylate kinase activity; ATP binding. Biological Process: dATP metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; ADP biosynthetic process; brain development; liver development; AMP metabolic process; oxidative phosphorylation; nucleotide phosphorylation. Disease: Reticular Dysgenesis

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

4345

more info or order :

MyBioSource product webpage