catalog number :
MBS143373
products type :
Recombinant Protein
products full name :
Recombinant Human Protein Phosphatase 1G
products short name :
Phosphatase 1G
products name syn :
PPM1G Human; Protein Phosphatase 1G Human Recombinant; Protein Phosphatase 1G; PP2CG; PPP2CG; MGC1675; MGC2870; PP2C GAMMA; EC 3.1.3.16; Protein phosphatase 2C isoform gamma; PP2C-gamma; Protein phosphatase magnesium-dependent 1 gamma; Protein phosphatase 1C; PPM1G; PPM1C
other names :
protein phosphatase 1G; Protein phosphatase 1G; protein phosphatase 1G; PP2C, gamma; PP2C-gamma; protein phosphatase 1C; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform; protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase 2C gamma isoform; protein phosphatase 2C isoform gamma; protein phosphatase magnesium-dependent 1 gamma; protein phosphatase, Mg2+/Mn2+ dependent, 1G; Protein phosphatase 1C; Protein phosphatase 2C isoform gamma; PP2C-gamma; Protein phosphatase magnesium-dependent 1 gamma
products gene name :
PPM1G
other gene names :
PPM1G; PPM1G; PP2CG; PPP2CG; PP2CGAMMA; PPM1C; PP2C-gamma
uniprot entry name :
PPM1G_HUMAN
sequence :
MGSSHHHHHH SSGLVPRGSH MEGKEEPGSD SGTTAVVALI RGKQLIVANA GDSRCVVSEA GKALDMSYDH KPEDEVELAR IKNAGGKVTM DGRVNGGLNL SRAIGDHFYK RNKNLPPEEQ MISALPDIKV LTLTDDHEFM VIACDGIWNV MSSQEVVDFI QSKISQRDEN GELRLLSSIV EELLDQCLAP DTSGDGTGCD NMTCIIICFK PRNTAELQPE SGKRKLEEVL STEGAEENGN SDKKKKAKRD.
purity :
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
The PPM1G solution (1mg/ml) contains 25mM Tris pH-7.5, 1mM DTT, 1mM EDTA, 2mM ?-ME and 20% glycerol. Sterile Filtered colorless solution.
storage stability :
PPM1G although stable 4 degree C for 4 weeks, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
products categories :
ENZYMES; Enzymes; Phosphatase
products description :
Description: PPM1G Human Recombinant fused with His-tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 250 amino acids and having a molecular mass of 27 kDa.The PPM1G is purified by proprietary chromatographic techniques. Introduction: PPM1G is part of the PP2C family of Ser/Thr protein phosphatases which are known to be negative regulators of cell stress response pathways. PPM1G is accountable for the dephosphorylation of Pre-mRNA splicing factors, an important factor for the formation of functional spliceosome. PPM1G regulates cell cycle progression. PPM1G mediates histone dephosphorylation/exchange in response to DNA damage or checkpoint recovery in higher eukaryotes. The degradation of p21/WAF1 induced by PPM1G is mediated in a proteasome-dependent manner. Protein phosphatase 1G regulates assembly and function of the beta-catenin degradation complex.
ncbi acc num :
NP_817092.1
ncbi gb acc num :
NM_177983.2
ncbi mol weight :
59,272 Da
ncbi pathways :
MRNA Processing Pathway 198843
ncbi summary :
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]