protein

Recombinant Human Dipeptidyl-Peptidase 4

MBS143360

0.002 mg

140 USD

Recombinant Protein

Recombinant Human Dipeptidyl-Peptidase 4

Dipeptidyl-Peptidase 4

DPP4 Human; Dipeptidyl-Peptidase 4 Human Recombinant; CD26; ADABP; ADCP2; DPPIV; TP103; DPP4; Dipeptidyl peptidase 4; Dipeptidyl peptidase IV; DPP IV; T-cell activation antigen CD26; Adenosine deaminase complexing protein 2; CD26 antigen

dipeptidyl peptidase 4; Dipeptidyl peptidase 4; dipeptidyl peptidase 4; ADCP-2; DPP IV; T-cell activation antigen CD26; adenosine deaminase complexing protein 2; dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); dipeptidyl-peptidase 4; ADABP; Adenosine deaminase complexing protein 2; ADCP-2; Dipeptidyl peptidase IV; DPP IV; T-cell activation antigen CD26; TP103; CD_antigen: CD26Cleaved into the following 2 chains:Dipeptidyl peptidase 4 membrane formAlternative name(s):Dipeptidyl peptidase IV membrane form

DPP4

DPP4; DPP4; CD26; ADABP; ADCP2; DPPIV; TP103; ADCP2; CD26; ADCP-2; DPP IV

DPP4_HUMAN

High-5 cells

766

ADP-[Mature form of DPP-4 (Ser 39 - Pro 766)]-SGRLVPRGSHHHHHH

Greater than 95.0% as determined by Analysis by SDS-PAGE.

DPP4 (0.5 mg/ml)is formulated in 20mM Tris-HCl buffer pH-8, 100mM NaCl, 1mM EDTA and 10% glycerol. Sterile Filtered colorless solution.

0.5 mg/ml

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1 HSA or BSA). Avoid multiple freeze/thaw cycles.

Assay Methods: - Reaction buffer: 20mM Tris pH8.0, 0.1M NaCl, 1mM EDTA- Total reaction volume: 100 ul- Reaction temperature: 37 degree C. 1. Add the reaction buffer to each well.2. Add the 10 ul of 10mM substrate (Gly-Pro p-nitroanilide) to each well.3. Add the enzyme (DPP-4) diluent to each well.4. Incubate the 96 well plate at 37 degree C.5. Read the optical density at 405 nm. Optical density was measured at 405 nm after incubating enzyme solution with 1mM of p-nitroanilid as a substrate. 0ng DPP4= 0 OD at 405nm.20ng DPP4= 0.075 OD at 405nm.40ng DPP4= 0.125 OD at 405nm.60ng DPP4= 0.2 OD at 405nm.80ng DPP4= 0.275 OD at 405nm.100ng DPP4= 0.35 OD at 405nm. Unit Defenition: One unit will hydrolyze 1 umole of p-nitroaniline per minute at pH8.0 at 37 degree C using 1mM of Gly-Pro p-nitroanilde as a substrate.

Biological Activity: >200 Units/mg.

ENZYMES; Enzymes; Peptidase

Description: DPPIV Human Recombinant produced in High-5 cells is a single, glycosylated polypeptide chain containing 746 amino acids (39-766) and having a molecular mass of 86.4 kDa.DPPIV is fused to His Tag at C-terminus and purified using conventional chromatography techniques. Introduction: DPP4 also called adenosine deaminase complexing protein-2, and T-cell activation antigen CD26 is a serine exopeptidase and complex enzyme that is expressed on the surface of most cell types. DPPIV is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP4 plays a role in t-cell activation. DPP4 is associated with intracellular signal transduction, apoptosis and involved in tumor biology. There are at least 63 substrates which can bind specifically to DPP4 enzyme including growth factors, chemokines, neuro peptides. Furthermore, DPP4 plays a major role in glucose metabolism by cleaving incretins such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1).

18765694

NP_001926.2

NM_001935.3

P27487

Incretin Synthesis, Secretion, And Inactivation Pathway (187170); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868); Synthesis, Secretion, And Inactivation Of Glucagon-like Peptide-1 (GLP-1) Pathway (187171); Synthesis, Secretion, And Inactivation Of Glucose-dependent Insulinotropic Polypeptide (GIP) Pathway (187172)

The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008]

DPP4: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF- kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. Belongs to the peptidase S9B family. DPPIV subfamily. Protein type: Cell adhesion; Membrane protein, integral; Cell surface; EC 3.4.14.5; Protease. Chromosomal Location of Human Ortholog: 2q24.3. Cellular Component: intercellular canaliculus; focal adhesion; cell surface; membrane; lysosomal membrane; endocytic vesicle; lamellipodium; apical plasma membrane; integral to membrane; plasma membrane; lipid raft. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protease binding; serine-type peptidase activity; dipeptidyl-peptidase activity; serine-type endopeptidase activity; receptor binding. Biological Process: regulation of cell-cell adhesion mediated by integrin; T cell activation; T cell costimulation; positive regulation of cell proliferation; response to hypoxia; endothelial cell migration; proteolysis

0.002 mg

140 USD

0.01 mg

205

1 mg

3870