Recombinant Human Klotho | MyBioSource MBS143354 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Klotho

catalog :

MBS143354

quantity :

0.005 mg

price :

140 USD

product information

catalog number :

MBS143354

products type :

Recombinant Protein

products full name :

Recombinant Human Klotho

products short name :

Klotho

products name syn :

KLOTHO Human; Klotho Human Recombinant; Klotho; KL

other names :

klotho; Klotho; klotho; klotho

products gene name :

KLOTHO

other gene names :

KL; KL

uniprot entry name :

KLOT_HUMAN

host :

Chinese Hamster Ovarian Cells (CHO)

sequence length :

1012

sequence :

EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSIKECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTI KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLlEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYHHHHH HH.

purity :

Greater than 90% as determined by SDS-PAGE.

form :

Klotho Human recombinant was filtered (0.4 um) and lyophilized from 0.5mg/ml in 0.08M phosphate buffer and 0.1M NaCl, pH 7.2. Physical Appearance: Filtered White lyophilized (freeze-dried) powder.

storage stability :

Store lyophilized Klotho Human recombinant at -20 degree C. Aliquot Klotho after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

other info2 :

Solubility: It is recommended to add to Klothodeionized water to prepare a working stock solution of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter Klothoby an appropriate sterile filter before using it in the cell culture.

products categories :

ENZYMES; Enzymes

products description :

Description: The Klotho Human Recombinant is produced in CHO cells and fused with a C-terminal 6xHis Tag. The Klotho His Tagged Fusion Protein is 59.5kDa protein containing a total of 522 amino acid residues. Introduction: Klotho is a type-I membrane protein which is related to beta-glucosidases. Reduced production of the Klotho protein is detected in patients with chronic renal failure (CRF), which may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. In addition, mutations within the Klotho protein are linked with ageing and bone loss.

ncbi gi num :

24497614

ncbi acc num :

NP_004786.2

ncbi gb acc num :

NM_004795.3

uniprot acc num :

Q9UEF7

ncbi mol weight :

62,135 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR Pathway (160957); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213307); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276)

ncbi summary :

This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]

uniprot summary :

Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; Membrane protein, integral; EC 3.2.1.31. Chromosomal Location of Human Ortholog: 13q12. Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; extracellular region; integral to membrane. Molecular Function: vitamin D binding; signal transducer activity; fibroblast growth factor binding; beta-glucosidase activity; beta-glucuronidase activity; hormone activity; fibroblast growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; calcium ion homeostasis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; carbohydrate metabolic process; insulin receptor signaling pathway; energy reserve metabolic process; innate immune response; positive regulation of bone mineralization; acute inflammatory response; aging. Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

0.5 mg

price3 :

2920