protein

Recombinant E Coli Carbonic Anhydrase 2

MBS143353

1 mg

2750 USD

Recombinant Protein

Recombinant E Coli Carbonic Anhydrase 2

Carbonic Anhydrase 2

Carbonic Anhydrase II E.coli; Carbonic Anhydrase II E.coli Recombinant; Carbonic anhydrase 2; Carbonate dehydratase 2; can; cynT2; yadF; b0126; JW0122; Carbonic Anhydrase II; Carbonic Anhydrase 2

carbonic anhydrase; Carbonic anhydrase 2; carbonic anhydrase; Carbonate dehydratase 2

can; can; ECK0125; JW0122; yadF; cynT2; yadF

CAN_ECOLI

E Coli

220

MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLAQAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL HIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK.

Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The Carbonic Anhydrase 2 enzyme is supplied in 20mM Tris pH-8 and 1mM DTT. Sterile Filtered colorless solution.

Carbonic Anhydrase II although stable at 4 degree C for 1 week, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

ENZYMES; Enzymes; Carbonic Anhydrase

Description: Carbonic anhydrase II is an E Coli Recombinant protein produced in E Coli containing 240 amino acids (1-220) and having a molecular mass of 27 kDa. Carbonic anhydrase is expressedwith an amino-terminal hexahistidine tag.The Carbonic anhydrase 2 is purified by proprietary chromatographic techniques. Introduction: The enzyme Carbonic anhydrase II having an accession number of NP_414668 is also called carbonate dehydratase which is part of the enzyme family that catalyses rapid inter-conversion of carbon dioxide & water to bicarbonate, carbonic acid and protons (CO2 + H2O ? HCO3? + H+), a reaction that occurs rather slowly in the absence of a catalyst. The majority of carbonic anhydrases enclose a zinc ion in their active site and therefore is classified as metalloenzymes.The most important function of Carbonic anhydrase is known to preserve acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Carbonic anhydrase has 3 different classes: alpha, beta and gamma which share very little sequence or structural similarity, thus far they all perform the same function and require a zinc ion at the active site. Mammalian carbonic anhydrase is monomeric and belongs to the alpha class. Plant carbonic anhydrase is dimeric and belongs to the beta class.Methane-producing bacteria carbonic anhydrase is trimeric and grows in hot springs which forms the gamma class.

16128119

NP_414668.1

NC_000913.3

P61517

25,097 Da

Nitrogen Metabolism Pathway 1101!!Nitrogen Metabolism Pathway 416

can forms a synthetic lethal with cynT, rescued by high CO2. [More information is available at EcoGene: EG12319]. Escherichia coli encodes two carbonic anhydrases, Can (carbonic anhydrase 2) and CynT (carbonic anhydrase 1). [More information is available at EcoCyc: EG12319].

1 mg

2750 USD