protein

Recombinant Human Galectin-1

MBS143303

0.01 mg

140 USD

Recombinant Protein

Recombinant Human Galectin-1

Galectin-1

LGALS1 Human; Galectin-1 Human Recombinant; Galectin-1; GAL1; GAL-1; Lectin galactoside-binding soluble 1; Beta-galactoside- binding lectin L-14-I; Lactose-binding lectin 1; S-Lac lectin 1; Galaptin; 14 kDa lectin; HPL; HBL; Putative MAPK-activating protein PM12; GBP; DKFZp686E23103

galectin-1; Galectin-1; galectin-1; 14 kDa laminin-binding protein; 14 kDa lectin; HBL; HLBP14; HPL; S-Lac lectin 1; beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa; gal-1; galaptin; galectin 1; lactose-binding lectin 1; putative MAPK-activating protein PM12; lectin, galactoside-binding, soluble, 1; 14 kDa laminin-binding protein; HLBP14; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; HBL; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; Putative MAPK-activating protein PM12; S-Lac lectin 1

LGALS1

LGALS1; LGALS1; GBP; GAL1; Gal-1; HLBP14

LEG1_HUMAN

E Coli

135

ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD.

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The Galectin-1 protein was lyophilized from a concentrated (1mg/ml) containing 10mM Na2PO4, pH-7.5. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Galectin-1 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Galectin-1 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized LGALS1 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: UV spectroscopy at 280 nm using the absorbency value of 0.59 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Biological Activity: Human Galectin-1 is a chemoattractant for human blood monocytes. ED50 range is from 0.053.0 ug/ml.

CYTOKINES AND GROWTH FACTORS; Growth Factors; Galectin

Description: LGALS1 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 14.5 kDa.The LGALS1 is purified by proprietary chromatographic techniques. Introduction: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 is an autocrine negative growth factor that regulates cell proliferation. Galectin-1 regulates cell apoptosis and cell differentiation. Galectin-1 binds CD45, CD3 and CD4 & inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of lyn kinase. Galectin-1 and its ligands are one of the master regulators of immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as hostpathogen interactions. Galectin-1 expression or overexpression in tumors and/or the tissue surrounding them must be considered as a sign of the malignant tumor progression that is often related to the long-range dissemination of tumoral cells (metastasis), to their dissemination into the surrounding normal tissue, and to tumor immune-escape. Galectin-1 in its oxidized form plays a number of important roles in the regeneration of the central nervous system after injury. The targeted overexpression (or delivery) of Galectin-1 should be considered as a method of choice for the treatment of some kinds of inflammation-related diseases, neurodegenerative pathologies and muscular dystrophies. In contrast, the targeted inhibition of Galectin-1 expression is what should be developed for therapeutic applications against cancer progression. Galectin-1 is thus a promising molecular target for the development of new and original therapeutic tools. There is 88% homology between the human and mouse galectin-1.

4504981

NP_002296.1

NM_002305.3

P09382

14,716 Da

Validated Targets Of C-MYC Transcriptional Repression Pathway (169353)

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]

Galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. Protein type: Cell adhesion. Chromosomal Location of Human Ortholog: 22q13.1. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; intracellular; nucleus. Molecular Function: signal transducer activity; protein binding; protein homodimerization activity; lactose binding; laminin binding; galactoside binding; glycoprotein binding. Biological Process: myoblast differentiation; regulation of apoptosis; response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; plasma cell differentiation; apoptosis; T cell costimulation; positive regulation of erythrocyte aggregation; response to axon injury; signal transduction; multicellular organismal response to stress

0.01 mg

140 USD

0.05 mg

205

1 mg

1365