protein

Recombinant Human Endothelial-Monocyte Activating Polypeptide II

MBS143297

0.02 mg

205 USD

Recombinant Protein

Recombinant Human Endothelial-Monocyte Activating Polypeptide II

Endothelial-Monocyte Activating Polypeptide II

EMAP II Human; Endothelial-Monocyte Activating Polypeptide II Human Recombinant; AIMP1; EMAP2; EMAP-2; EMAPII; SCYE1; Multisynthetase complex auxiliary component p43; Endothelial monocyte-activating polypeptide 2; EMAP-II; p43

aminoacyl tRNA synthase complex-interacting multifunctional protein 1 isoform a; Aminoacyl tRNA synthase complex-interacting multifunctional protein 1; aminoacyl tRNA synthase complex-interacting multifunctional protein 1; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; endothelial-monocyte activating polypeptide II; multisynthase complex auxiliary component p43; multisynthetase complex auxiliary component p43; small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating); aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; Multisynthase complex auxiliary component p43Endothelial monocyte-activating polypeptide 2; EMAP-2Alternative name(s):Endothelial monocyte-activating polypeptide II; EMAP-II; Small inducible cytokine subfamily E member 1

EMAP II

AIMP1; AIMP1; p43; HLD3; EMAP2; SCYE1; EMAPII; EMAP2; SCYE1; EMAP-2; EMAP-II

AIMP1_HUMAN

E Coli

312

SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQM QNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK.

Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM sodium Phosphate buffer pH=7.5 and 130mM sodium chloride. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized EMAP-II although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution EMAP-II should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized EMAP-II in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: Determined by the apoptotic effect on MCF-7 cells using a concentration of 20-30 ng/ml.

CYTOKINES AND GROWTH FACTORS; Cytokines

Description: EMAP-II Recombinant Human produced in E Coli is a single, non-glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of 18.3 kDa. The EMAP-II is purified by proprietary chromatographic techniques. Introduction: EMAP-II also called SCYE1 is a tumor derived cytokine that plays a role in a wide variety of activities on endothelial cells, monocytes and neutrophils. EMAP-II inhibits endothelial cell proliferation, vasculogenesis, neovessel formation, and can induce apoptosis. It is also chemotactic towards neutrophils and monocytes and induces myeloperoxidase activity from neutrophils. EMAP-II clinical value is inhibiting angiogenesis of vascular beds and suppressing the growth of primary and secondary tumors with no affect to normal tissues. SCYE1is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this SCYE1 renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. EMAP-2 plays a role in in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells.

215490009

NP_001135887.1

NM_001142415.1

Q12904

37,039 Da

Cytosolic TRNA Aminoacylation Pathway 105981!!Gene Expression Pathway 105937!!TRNA Aminoacylation Pathway 105980

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq, Dec 2008]

0.02 mg

205 USD