Recombinant Human Apolipoprotein-D | MyBioSource MBS143287 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Apolipoprotein-D

catalog :

MBS143287

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS143287

products type :

Recombinant Protein

products full name :

Recombinant Human Apolipoprotein-D

products short name :

Apolipoprotein-D

products name syn :

Apo D Human; Apolipoprotein-D Human Recombinant; Apolipoprotein D; Apo-D; ApoD

other names :

apolipoprotein D; Apolipoprotein D; apolipoprotein D; apo-D; apolipoprotein D

products gene name :

APOD

other gene names :

APOD; APOD; Apo-D; ApoD

uniprot entry name :

APOD_HUMAN

host :

E Coli

sequence length :

189

sequence :

FHLGKCPNPP VQENFDVNKY PGRWYEIEKI PTTFENGRCI QANYSLMENG KIKVLNQELR ADGTVNQIEG EATPVNLTEP AKLEVKFSWF MPSAPYHILA TDYENYALVY SCTSISQSFH VDFAWILARN VALPPETVDS LKNILTSNNI DVKKMTVTDQ VNCPKLSAHHHHHH.

purity :

Greater than 95% as determined by SDS-PAGE.

form :

Filtered (0.4 um) and lyophilized from 1mg/ml in 4mM KH2PO4, 16mM Na2HPO4 and 115mM NaCl pH 7.5. Filtered White lyophilized (freeze-dried) powder.

storage stability :

Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

other info2 :

Solubility: It is recommended to add deionized H2O to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter this product by an appropriate sterile filter before using it in the cell culture.

products categories :

CYTOKINES AND GROWTH FACTORS; Cytokines; Apolipoprotein

products description :

Description: Apolipoprotein-D Human Recombinant His Tag fusion protein at C-terminus (7 highlighted a.a.) produced in E Coli is a single, non-glycosylated, Polypeptide chain containing 174 amino acids and having a molecular mass of 19.82kDa. The protein a.a sequence corresponds to the UniProtKB/Swiss-Prot entry P05090.The Following gene modifications were made:Trp99His, Cys116Ser, Ile118Ser, Leu120Ser amino acids exchanges were introduced at the surface of Apolipoprotein-D to enhance the protein's solubility and another three Leu23Pro, Pro133Val, Asn134Ala amino acids exchanges which facilitate its genetic manipulation. The Apolipoprotein-D is purified by proprietary chromatographic techniques. Introduction: Apolipoprotein-D is mainly associated with high density lipoproteins in human plasma. Apolipoprotein-D is an atypical apolipoprotein and, based on its primary structure, Apolipoprotein-D is a member of the lipocalin family. Lipocalins adopt a beta-barrel tertiary structure and transport small hydrophobic ligands. Apolipoprotein-D binds cholesterol, progesterone, pregnenolone, bilirubin and arachidonic acid. Apolipoprotein-D is expressed in numerous tissues having high levels of expression in spleen, testes and brain. Apolipoprotein-D is present at high concentrations in the cyst fluid of women with gross cystic disease of the breast, a condition associated with increased risk of breast cancer. Apolipoprotein-D accumulates in regenerating peripheral nerves and in the cerebrospinal fluid of patients with neurodegenerative conditions, such as Alzheimer's disease. Apolipoprotein-D participates in maintenance and repair within the central and peripheral nervous systems. Apolipoprotein-D is a multi-ligand, multi-functional transporter and transports a ligand from 1 cell to another within an organ, scavenge a ligand within an organ for transport to the blood or could transport a ligand from the circulation to specific cells within a tissue.

ncbi gi num :

4502163

ncbi acc num :

NP_001638.1

ncbi gb acc num :

NM_001647.3

uniprot acc num :

P05090

ncbi mol weight :

21,276 Da

ncbi pathways :

Orphan Transporters Pathway (1127546); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Fatty Acids Pathway (187185); Transport Of Vitamins, Nucleosides, And Related Molecules Pathway (161075)

ncbi summary :

This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq, Aug 2008]

uniprot summary :

APOD: APOD occurs in the macromolecular complex with lecithin- cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts. Belongs to the calycin superfamily. Lipocalin family. Protein type: Secreted; Lipid-binding; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q29. Cellular Component: extracellular space; cell soma; endoplasmic reticulum; perinuclear region of cytoplasm; dendrite; extracellular region. Molecular Function: lipid transporter activity; protein binding; cholesterol binding. Biological Process: response to drug; negative regulation of smooth muscle cell proliferation; negative regulation of focal adhesion formation; glucose metabolic process; negative regulation of protein import into nucleus; lipid transport; axon regeneration in the peripheral nervous system; response to reactive oxygen species; tissue regeneration; response to axon injury; brain development; lipid metabolic process; angiogenesis; aging

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

5730

more info or order :

MyBioSource product webpage