catalog number :
MBS143255
products type :
Recombinant Protein
products full name :
Recombinant Tobacco Etch Virus Protease
products short name :
Tobacco Etch Virus Protease
products name syn :
TEV; Tobacco Etch Virus Protease Recombinant; rTEV; TEV; P1 protease
other names :
P1 protease; P1 protease; P1 protease
uniprot entry name :
Q0GDU8_TEV
purity :
Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
The rTEV contains 0.50M Tris-HCl pH 8.0, 10mM DTT and 5mM EDTA. Sterile liquid formulation.
storage stability :
rTEV although stable at 4 degree C for 1 week, should be stored below -18 degree C. Please prevent freeze thaw cycles.
other info1 :
Cleavage Conditions: A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4 degree C incubations are recommended. Reactions at 4 degree C will require longer incubation times and/or more rTEV.
other info2 :
Activity: 10,000 Units/1mg.
products categories :
RECOMBINANT & NATURAL PROTEINS; Enzymes; Protease
products description :
Description: Recombinant TEV Protease (rTEV) is a site-specific protease purified from E Coli. The protease can be used for the removal of affinity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30 degree C; however, the enzyme can be used at temperatures as low as 4 degree C. The rTEV contains His tag.The rTEV is purified by proprietary chromatographic techniques. Introduction: TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G/S), with cleavage occurring between Q and G or Q and S. The most commonly used sequence is ENLYFQG.
ncbi mol weight :
34,786 Da