Recombinant Human Thiopurine S-methyltransferase | MyBioSource MBS143244 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Thiopurine S-methyltransferase

catalog :

MBS143244

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS143244

products type :

Recombinant Protein

products full name :

Recombinant Human Thiopurine S-methyltransferase

products short name :

Thiopurine S-methyltransferase

products name syn :

TPMT Human; Thiopurine S-methyltransferase Human Recombinant; TPMT; Thiopurine S-methyltransferase; EC 2.1.1.67; Thiopurine methyltransferase

other names :

thiopurine S-methyltransferase; Thiopurine S-methyltransferase; thiopurine S-methyltransferase; S-adenosyl-L-methionine:thiopurine S-methyltransferase; thiopurine S-methyltransferase; Thiopurine methyltransferase

products gene name :

TPMT

other gene names :

TPMT; TPMT

uniprot entry name :

TPMT_HUMAN

host :

E Coli

sequence length :

245

sequence :

MDGTRTSLDI EEYSDTEVQK NQVLTLEEWQ DKWVNGKTAF HQEQGHQLLK KHLDTFLKGKSGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIQEFF TEQNLSYSEE PITEIPGTKVFKSSSGNISL YCCSIFDLPR TNIGKFDMIW DRGALVAINP GDRKCYADTM FSLLGKKFQY LLCVLSYDPT KHPGPPFYVP HAEIERLFGK ICNIRRLEKV DAFEERHKSW GIDCLFEKLYLLTEK.

purity :

Greater than 95.0% as determined by SDS-PAGE.

form :

1 mg/ml solution containing 20mM Tris 8.0, 0.2mM PMSF and 2mM EDTA. Sterile filtered colorless solution.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

products categories :

PROTEIN KINASES; Enzymes; Protein Kinases

products description :

Description: TPMT Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 245 amino acids (1-245) and having a molecular mass of 28 kDa. Thiopurine S-methyltransferase is purified by proprietary chromatographic techniques. Introduction: TPMT, thiopurine S-methyltransferase, is a cytosolic enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. TPMT activity exhibits autosomal codominant genetic polymorphism, and patients inheriting TPMT-deficiency are at high risk of potentially fatal hematopoietic toxicity.

ncbi gi num :

4507653

ncbi acc num :

NP_000358.1

ncbi gb acc num :

NM_000367.3

uniprot acc num :

P51580

ncbi mol weight :

28,180 Da

ncbi pathways :

Biological Oxidations Pathway (105698); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1127658); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1127659); Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway (1127660); Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway (1127663); Defective OPLAH Causes 5-oxoprolinase Deficiency (OPLAHD) Pathway (1127661); Defective SLC35D1 Causes Schneckenbecken Dysplasia (SCHBCKD) Pathway (1127665); Defective TPMT Causes Thiopurine S-methyltransferase Deficiency (TPMT Deficiency) Pathway (1127666); Defective UGT1A1 Causes Hyperbilirubinemia Pathway (1127667)

ncbi summary :

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014]

uniprot summary :

TPMT: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. Defects in TPMT are the cause of thiopurine S- methyltransferase deficiency (TPMT deficiency). TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus. Belongs to the methyltransferase superfamily. TPMT family. Protein type: EC 2.1.1.67; Xenobiotic Metabolism - drug metabolism - other enzymes; Methyltransferase. Chromosomal Location of Human Ortholog: 6p22.3. Cellular Component: cytosol. Molecular Function: thiopurine S-methyltransferase activity. Biological Process: methylation; xenobiotic metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process. Disease: Thiopurine S-methyltransferase Deficiency

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

3490

more info or order :

MyBioSource product webpage