catalog number :
MBS143213
products type :
Recombinant Protein
products full name :
Recombinant HIV-1 p31 Integrase
products short name :
HIV-1 p31 Integrase
products name syn :
HIV-1 Integrase p31; HIV-1 Integrase p31 Recombinant; HIV-1 Integrase
specificity :
Immunoreactive with all sera of HIV-1 infected individuals.
purity :
Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.
form :
1.5M urea, 25mM Tris-HCl pH 8.0, 0.2% Triton-X & 50% Glycerol. Sterile filtered colorless clear solution.
tested application :
ELISA (EIA), Western Blot (WB)
app notes :
HIV-1 Integrase p31 antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
products categories :
VIRAL ANTIGENS- HUMAN IMMUNODEFICIENCY VIRUS; Viral Antigens; HIV
products description :
Description: The E Coli derived recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 immunodominant regions from the p31 protein (integrase) 9-289 amino acids, fused with six histidines at N-terminus. Introduction: Integrase is an enzyme produced by the HIV which enables its genetic material to be integrated into the DNA of the infected cell and is a key component in the pre-integration complex. HIV integrase contains 3 domains, an N-terminal HH-CC zinc fingerdomainwhich is partially responsible for multimerization, a central catalytic domain and a C-terminal domain. Both Central catalytic domain and C-terminal domains have been shown to bind both viral and cellular DNA. No crystal structure data exists with Integrase bound to its DNA substrates. HIV-1 integrase functions as a dimeror a tetramer. Additionally, several host cellular proteins interact with integrase and may facilitate the integration process.