product summary
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company name :
MyBioSource
product type :
protein
product name :
Recombinant Protease
catalog :
MBS143200
quantity :
50 IU
price :
140 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS143200
products type :
Recombinant Protein
products full name :
Recombinant Protease
products short name :
Protease
products name syn :
Protease; Recombinant Protease; Protease
other names :
protease; Tricorn protease homolog 1; protease
other gene names :
SCO2549; tri1; SCC77.16c.
uniprot entry name :
TRI1_STRCO
host :
E Coli
sequence length :
1067
form :
Sterile filtered colorless solution.
concentration :
1000IU/1.43ml
storage stability :
Protease Recombinant although stable at 4 degree C for 1 week, should be stored desiccated below -18 degree C. Please prevent freeze thaw cycles.
other info1 :
Cleavage Buffer: 50mM Tris-HCl, pH-7.0 (at 25 degree C), 150mM NaCl, 1mM EDTA, 1mM dithiothreitol. Chill to 5 degree C prior to use. Cleavage Conditions: For Cleavage of a Fusion Protein: During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20, NonidetTM, or NP40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit PreScission Protease. Unit Defenition: One unit will cleave ?90% of 100 ug of a test GST-fusion protein in Cleavage Buffer (50mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0 at 25 degree C) at 5 degree C for 16 hours.
products categories :
ENZYMES; Enzymes; Protease
products description :
Description: Protease Recombinant is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well.The Recombinant Protease is purified by proprietary chromatographic techniques.
ncbi gi num :
21221008
ncbi acc num :
NP_626787.1
ncbi gb acc num :
NC_003888.3
ncbi mol weight :
115,647 Da
size1 :
50 IU
price1 :
140 USD
size2 :
150 IU
price2 :
205
size3 :
1000 IU
price3 :
455
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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