protein

Recombinant Human Lipopolysaccarid Binding Protein

MBS143089

0.01 mg

610 USD

Recombinant Protein

Recombinant Human Lipopolysaccarid Binding Protein

Lipopolysaccarid

LBP Human; Lipopolysaccaride Human Recombinant; Lipopolysaccharide-binding protein; LBP; MGC22233

lipopolysaccharide-binding protein; Lipopolysaccharide-binding protein; lipopolysaccharide-binding protein; BPI fold containing family D, member 2; LPS-binding protein; lipopolysaccharide binding protein

LBP

LBP; LBP; BPIFD2; LBP

LBP_HUMAN

Chinese Hamster Ovarian Cells (CHO)

481

Recombinant Human LBP was lyophilized from a protein solution (0.25 mg/ml) containing phosphate-buffered saline, pH 7.2. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized LBP Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution LBP should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.

Characterization: On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa.

Solubility: Every 10 ug of recombinant human LBP should be reconstituted using 40 ul of sterile H2O. The solution can be than diluted with phosphate-buffered saline or other buffers. Activity: Up to 0.2 ug/ ml LBP mediates binding of FITC-LPS (0.5 ug/ml) to CD14+CHO transfectants (FACS).

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Lipopolysaccarid Binding Protein

Description: The Lipopolysaccharide Binding Protein is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.The recombinant Human LBP was purified by his-tag with metal affinity purification with Talon and controlled by SDS page.Attention: His-tag has no protease site and can not be split off. Introduction: Lipopolysaccharides (LPS) are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein (aka LBP) is a plasma protein which facilitates the diffusion of bacterial LPS (endotoxin). LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein (BPI), LBP binds LPS and interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, and to a binding site on soluble CD14 (sCD14). sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, and then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid.

31652249

NP_004130.2

NM_004139.4

P18428

53,384 Da

IL6-mediated Signaling Events Pathway 137932!!Immune System Pathway 106386!!Innate Immune System Pathway 106387!!NF-kappa B Signaling Pathway 634527!!Regulation Of Toll-like Receptor Signaling Pathway 920955!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149!!Toll Like Receptor 4 (TLR4) Cascade Pathway 106398!!Toll-Like Receptors Cascades Pathway 106388!!Toll-like Receptor Signaling Pathway 83076

The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012]

0.01 mg

610 USD