protein

Recombinant Human CD14 CHO

MBS143087

0.01 mg

140 USD

Recombinant Protein

Recombinant Human CD14 CHO

CD14 CHO

CD14 Human, CHO; CD14 Human Recombinant, CHO; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14; CD14 CHO

monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; CD14 molecule; Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14Cleaved into the following 2 chains:Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form

CD14

CD14; CD14

CD14_HUMAN

Chinese Hamster Ovarian Cells (CHO)

375

Greater than 95.0% as determined by SDS-PAGE.

CD14 was lyophilized from a concentrated protein solution (1.0 mg/ml) containing phosphate-buffered saline, pH 7.2. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CD14 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized CD14 in sterile 18M Omega -cm H2O not less than 100 ug/ml. Further dilutions should be made with phosphate buffered saline (PBS). Biological Activity: Up to 20 ug/ml CD14 inhibit binding of FITC-LPS (0.5 ug/ml) to 600,000 CD14+CHO transfectants (FACS).

RECOMBINANT & NATURAL PROTEINS; CD Antigens

Description: The CD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50-kDa protein containing 335 amino acids and lacks the GPI-Anchor. Introduction: CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.

4557417

NP_000582.1

NM_000591.3

P08571

40,076 Da

Activated TLR4 Signalling Pathway 106400!!Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway 187088!!Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489!!IKK Complex Recruitment Mediated By RIP1 Pathway 187090!!Immune System Pathway 106386!!Innate Immune System Pathway 106387!!Legionellosis Pathway 469200

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]

0.01 mg

140 USD