Bovine Vimentin | MyBioSource MBS143077 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Bovine Vimentin

catalog :

MBS143077

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS143077

products type :

Native Protein

products full name :

Bovine Vimentin

products short name :

Vimentin

products name syn :

Vimentin Bovine; Bovine Vimentin; Vimentin; Vim; FLJ36605; bVimentin

other names :

vimentin; Vimentin; vimentin; vimentin

other gene names :

VIM; VIM

uniprot entry name :

VIME_BOVIN

host :

Bovine Lens

sequence length :

466

purity :

Greater than 98.0% as determined by SDS-PAGE.

form :

The protein (1mg/ml) was lyophilized from a 1mg/ml solution containing 10mM sodium phosphate pH 7.5, 6M urea, 2mM DTT, 1mM EDTA and 10mM methylammonium chloride. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized Bovine Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution BovineVimentin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the Bovine Vimentin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Reconstitution: After vimentin is dissolved in 6M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Sodium Phosphate, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco''s PBS).- Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841.- Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255.

products categories :

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Vimentin

products description :

Description: Bovine Vimentin protein produced in Bovine Lens, having a molecular weight of 57 kDa. Introduction: Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms.

ncbi gi num :

110347570

ncbi acc num :

NP_776394.2

ncbi gb acc num :

NM_173969.3

uniprot acc num :

P48616

ncbi mol weight :

53,728 Da

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway (920929); Apoptosis Pathway (1119590); Apoptotic Cleavage Of Cellular Proteins Pathway (1119612); Apoptotic Execution Phase Pathway (1119611); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (1119613); Epstein-Barr Virus Infection Pathway (585566); Epstein-Barr Virus Infection Pathway (587115); MicroRNAs In Cancer Pathway (852708); MicroRNAs In Cancer Pathway (852928); Muscle Contraction Pathway (1120018)

uniprot summary :

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Cellular Component: neuron projection; focal adhesion; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol. Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens. Biological Process: Bergmann glial cell differentiation; in utero embryonic development; intermediate filament organization; astrocyte development

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

3490

more info or order :

MyBioSource product webpage