protein

Filamin

MBS143073

0.01 mg

140 USD

Native Protein

Filamin

Filamin

Filamin; Filamin; Filamin

filamin; Filamin-A; filamin-A; actin binding protein 280; alpha-filamin; endothelial actin-binding protein; filamin-1; non-muscle filamin; filamin A, alpha; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin

FLNA; FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280; FLN; FLN1; FLN-A; ABP-280

FLNA_HUMAN

Chicken Gizzard

2647

Greater than 90.0% as determined by SDS-PAGE.

The protein was lyophilized from a 1mg/ml solution containing 20mM Tris / acetate buffer pH 7.6, 0.1mM EDTA, 2mM DTT and 20mM NaCl. Each mg Filamin contains 550mg urea. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Filamin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Filamin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Filamin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

RECOMBINANT & NATURAL PROTEINS; Natural Proteins

Description: Ultra Pure Filamin having a Molecular mass of 250 kDa. Introduction: Filamin is a large (270kd) dimeric actin crosslinking protein from a variety of sources, which helps to stabilize the 3D cortical actin network. The fundamental structure of filamin is well conserved and consists of an actin binding domain at the N-terminus followed by a C-terminal rod domain consisting of numerous repeat segments ranging from 4 in C. elegans to 24 in mammalian cells. Each repeat in the rod domain consists of roughly 100 residues and forms an immunoglobulin like fold. Such immunoglobulin folds have been found in a variety of proteins and are responsible for protein-protein interactions. Filamin Human actin-binding protein (ABP), aka filamin, crosslinks actin filaments into orthogonal networks in cortical cytoplasm and participates in the anchoring of membrane proteins for the actin cytoskeleton. Mammalian filamin interacts directly with at least 30 proteins such as transmembrane receptors, second messenger-associated proteins, protein kinases, phosphatases and cytoskeletal proteins and these interactions have been shown to require one or more of the repeat elements in the rod domain.

1203969

AAA92644.1

280,018 Da

Androgen Receptor Signaling Pathway (198806); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-extracellular Matrix Interactions Pathway (160967); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); GP1b-IX-V Activation Signalling Pathway (187205); Hemostasis Pathway (106028); MAPK Signaling Pathway (198779)

The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

FLNA: a ubiquitous cytoskeletal protein that promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Plays an essential role in embryonic cell migration. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interactions with filamin A may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking. Protein type: Motility/polarity/chemotaxis; Actin-binding; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: Xq28. Cellular Component: cortical cytoskeleton; focal adhesion; extracellular region; actin filament; trans-Golgi network; cytosol; actin cytoskeleton; membrane; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus. Molecular Function: small GTPase binding; actin filament binding; signal transducer activity; protein binding; Rho GTPase binding; protein homodimerization activity; protein kinase C binding; mu-type opioid receptor binding; Rac GTPase binding; Ral GTPase binding; glycoprotein binding; SMAD binding; transcription factor binding. Biological Process: actin crosslink formation; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; negative regulation of transcription factor activity; mRNA transcription from RNA polymerase II promoter; receptor clustering; dopamine receptor, adenylate cyclase inhibiting pathway; establishment of protein localization; platelet degranulation; early endosome to late endosome transport; actin cytoskeleton reorganization; positive regulation of transcription factor import into nucleus; cytoplasmic sequestering of protein; epithelial to mesenchymal transition; cilium biogenesis; blood coagulation; negative regulation of protein catabolic process. Disease: Terminal Osseous Dysplasia; Fg Syndrome 2; Cardiac Valvular Dysplasia, X-linked; Frontometaphyseal Dysplasia; Melnick-needles Syndrome; Heterotopia, Periventricular, Ehlers-danlos Variant; Intestinal Pseudoobstruction, Neuronal, Chronic Idiopathic, X-linked; Heterotopia, Periventricular, X-linked Dominant; Otopalatodigital Syndrome, Type Ii; Otopalatodigital Syndrome, Type I

0.01 mg

140 USD

0.05 mg

205

1 mg

1415