catalog number :
MBS143070
products type :
Native Protein
products full name :
Alpha Actinin
products short name :
Alpha Actinin
products name syn :
a-Actinin; Actinin Alpha; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; Non-muscle alpha-actinin-1; F-actin cross-linking protein; ACTN1; Alpha Actinin
other names :
alpha-actinin-1; Alpha-actinin-1; alpha-actinin-1; F-actin cross-linking protein; alpha-actinin cytoskeletal isoform; non-muscle alpha-actinin-1; actinin, alpha 1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1
other gene names :
ACTN1; ACTN1
uniprot entry name :
ACTN1_CHICK
purity :
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
The protein was lyophilized from a 1mg/ml solution containing 10mM Tris acetate buffer pH 7.6, 0.1mM EDTA, 2mM DTT, and 20mM NaCl. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized a-Actinin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution a-Actinin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
app notes :
Protein standard in 1D and 2D SDS gelelectrophoresisImmunoassays Immunization.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized a-Actinin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
products categories :
RECOMBINANT & NATURAL PROTEINS; Natural Proteins
products description :
Description: Ultra pure Alpha Actinin having a Molecular mass of 95,000 Dalton. Introduction: ACTN1 encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
ncbi acc num :
NP_989458.1
ncbi gb acc num :
NM_204127.1
ncbi mol weight :
102,855 Da
ncbi pathways :
Adherens Junction Pathway (119315); Adherens Junction Pathway (481); Focal Adhesion Pathway (119312); Focal Adhesion Pathway (478); Regulation Of Actin Cytoskeleton Pathway (672477); Regulation Of Actin Cytoskeleton Pathway (119319); Regulation Of Actin Cytoskeleton Pathway (500); Tight Junction Pathway (119316); Tight Junction Pathway (482)